Literature summary for 4.6.1.20 extracted from

Noguchi, S.
Structural changes induced by the deamidation and isomerization of asparagine revealed by the crystal structure of Ustilago sphaerogena ribonuclease U2B (2010), Biopolymers, 93, 1003-1010.

Crystallization (Commentary)

Crystallization (Comment)	Organism
isoAsp containing ribonuclease U2B, hanging drop vapor diffusion method, 0.004 ml of 10 mg/ml protein in 50 mM sodium citrate, pH 4.5, containing 50 mM sodium chloride, are mixed with 0.004 ml of reservoir solution containing 0.84 M sodium dihydrogen phosphate and 1.26 M dipotassium hydrogen phosphate, pH 6.9, equilibration against 0.4 ml reservoir solution, 20°C, 2 days, X-ray diffraction structure determination and analysis at 1.32 A resolution	Ustilago sphaerogena

Crystallization (Comment)

Organism

isoAsp containing ribonuclease U2B, hanging drop vapor diffusion method, 0.004 ml of 10 mg/ml protein in 50 mM sodium citrate, pH 4.5, containing 50 mM sodium chloride, are mixed with 0.004 ml of reservoir solution containing 0.84 M sodium dihydrogen phosphate and 1.26 M dipotassium hydrogen phosphate, pH 6.9, equilibration against 0.4 ml reservoir solution, 20°C, 2 days, X-ray diffraction structure determination and analysis at 1.32 A resolution

Ustilago sphaerogena

Organism

Organism	UniProt	Comment	Textmining
Ustilago sphaerogena	P00654	-	-

Purification (Commentary)

Purification (Comment)	Organism
native RNase U2A by anion-exchange and 5'-adenylate aminohexyl affinity chromatography	Ustilago sphaerogena

Subunits

Subunits	Comment	Organism
More	formation of isoAsp32 induces a single turn unfolding of the alpha-helix from Asp29 to Asp34, and the region from Asp29 to Arg35 forms a U-shaped loop structure, overview	Ustilago sphaerogena

Synonyms

Synonyms	Comment	Organism
ribonuclease U2B	-	Ustilago sphaerogena

General Information

General Information	Comment	Organism
additional information	formation of isoAsp32 induces a single turn unfolding of the alpha-helix from Asp29 to Asp34, and the region from Asp29 to Arg35 forms a U-shaped loop structure, overview. IsoAsp32 protrudes from the surface of the protein, and the abnormal beta-peptide bond in the main-chain and alpha-carboxylate in the side-chain is fully exposed. The structure suggests that the deamidation of the Asn and the isoAsp formation in proteins could confer immunogenicity	Ustilago sphaerogena