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Literature summary for 4.6.1.20 extracted from

  • Martinez-Ruiz, A.; Garcia-Ortega, L.; Kao, R.; Lacadena, J.; Onaderra, M.; Mancheno, J.M.; Davies, J.; Del Pozo, A.M.; Gavilanes, J.G.
    RNase U2 and alpha-sarcin: a study of relationships (2001), Methods Enzymol., 341, 335-351.
    View publication on PubMed
Search for more literature for 4.6.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Ustilago sphaerogena

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12490
-
-
 Ustilago sphaerogena

Organism

Organism UniProt Comment Textmining
Ustilago sphaerogena P00654 ATCC 12421 and CBS 534.71
-

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant enzyme using several chromatographic steps, including DEAE-cellulose and affinity chromatography on 2'-5'-ADP-Sepharose Ustilago sphaerogena

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA + H2O
-
 Ustilago sphaerogena 3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
 ?

Synonyms

Synonyms Comment Organism
RNase U2
-
 Ustilago sphaerogena

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Ustilago sphaerogena

pI Value

Organism Comment pI Value Maximum pI Value
Ustilago sphaerogena
-
 4.8 4.7