Application | Comment | Organism |
---|---|---|
additional information | cGMP suppresses pseudopod formation in the back of the cell, whereas the sGC protein refines pseudopod formation at the leading edge | Dictyostelium discoideum |
Cloned (Comment) | Organism |
---|---|
wild-type sGC and mutant sGC constructs fused at the C-terminus to GFP and expressed in gc-null cells | Dictyostelium discoideum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant sGCdeltacat with point mutation introduced in the catalytic site, loses catalytic activity, shows poor myosin localization at the back, but excellent localization of the sGC protein at the leading edge, where it enhances the probability that a new pseudopod is made in proximity to previous pseudopodia, resulting in a decrease of the degree of turning. Mutant sGCdeltaN with deletion of the N-terminal 877 amino acids, exhibits excellent cGMP formation and myosin localization in the back of the cell, but exhibits poor orientation at the leading edge | Dictyostelium discoideum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | Q963L5 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pseudopodium | sGC protein enriched at the leading edge of the cell during chemotaxis | Dictyostelium discoideum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | - |
Dictyostelium discoideum | 3',5'-cyclic GMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sGC | - |
Dictyostelium discoideum |
soluble guanylyl cyclase | - |
Dictyostelium discoideum |