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Literature summary for 4.6.1.2 extracted from

  • Winger, J.A.; Derbyshire, E.R.; Marletta, M.A.
    Dissociation of nitric oxide from soluble guanylate cyclase and heme-nitric oxide/oxygen binding domain constructs (2007), J. Biol. Chem., 282, 897-907.
    View publication on PubMed
Search for more literature for 4.6.1.2

Activating Compound

Activating Compound Comment Organism Structure
(Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate sGC ferrous-nitrosyl complex adopts two 5-coordinate conformations, a lower activity closed form, which releases NO slowly, and a higher activity open form, which releases NO rapidly Rattus norvegicus
3-(5'-hydroxymethyl-3'-furyl)-1-benzylindazole
-
 Rattus norvegicus

Application

Application Comment Organism
additional information sGC has two NO-regulated activity states Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
sGC beta1 cDNA inserted between the NotI and XbaI sites of the plasmid pFastBac1, H6sGC alpha1 gene inserted between the NotI and XbaI sites of pFastBac1, expression in Sf9 cells Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on Ni2+ column and anion-exchange column Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanosine-5'-[alpha,beta-methylene]triphosphate
-
 Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
sGC
-
 Rattus norvegicus
soluble guanylate cyclase
-
 Rattus norvegicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 37 inclusion of 3-(5'-hydroxymethyl-3'-furyl)-1-benzylindazole moderately accelerates NO dissociation from sGC and beta2(1-217) at 37°C and dramatically accelerates NO dissociation from sGC at 10°C. Presence of guanosine-5'-[alpha,beta-methylene]triphosphate also dramatically accelerates NO dissociation from sGC at 10 °C Rattus norvegicus