Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NO | is bound in the distal heme pocket of the L2 H-NOX fold, NO dissociates from the distal side of the heme in both 5- and 6-coordinate complexes | Legionella pneumophila | |
NO | is bound in the distal heme pocket of the L2 H-NOX fold, NO dissociates from the distal side of the heme in both 5- and 6-coordinate complexes | Nostoc punctiforme |
Cloned (Comment) | Organism |
---|---|
into pET-20b vector | Legionella pneumophila |
into pET-20b vector | Nostoc punctiforme |
Protein Variants | Comment | Organism |
---|---|---|
F142Y | L2 H-NOX mutant, shift of the equilibrium of the FeII-NO complex at 20°C exclusively to the 6-coordinate complex | Legionella pneumophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | forms a temperature-dependent mixture of 5- and 6-coordinate FeII-NO complexes | Nostoc punctiforme | |
Fe2+ | L1 H-NOX forms a temperature-independent 5-coordinate FeII-NO complex, L2 H-NOX forms a temperature-dependent mixture of 5- and 6-coordinate FeII-NO complexes | Legionella pneumophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Legionella pneumophila | - |
- |
- |
Nostoc punctiforme | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Nostoc punctiforme |
by metal affinity chromatography followed by gel filtration | Legionella pneumophila |
Synonyms | Comment | Organism |
---|---|---|
H-NOX | - |
Nostoc punctiforme |
L1 H-NOX | - |
Legionella pneumophila |
L2 H-NOX | - |
Legionella pneumophila |
sGC | - |
Legionella pneumophila |
sGC | - |
Nostoc punctiforme |
soluble guanylate cyclase | - |
Legionella pneumophila |
soluble guanylate cyclase | - |
Nostoc punctiforme |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
L2 H-NOX is a mixture of 5- and 6-coordinate complexes under room temperature conditions, at low temperature, it is primarily 6-coordinate, and at high temperature, there is a shift toward the 5-coordinate geometry | Legionella pneumophila |