Literature summary for 4.6.1.2 extracted from

Yazawa, S.; Tsuchiya, H.; Hori, H.; Makino, R.
Functional characterization of two nucleotide-binding sites in soluble guanylate cyclase (2006), J. Biol. Chem., 281, 21763-21770.

Search for more literature for 4.6.1.2

Activating Compound

Activating Compound	Comment	Organism	Structure
BAY 41-2272	one molecule is required for maximal enzyme activation and is tightly associated with sGC	Bos taurus

Application

Application	Comment	Organism
additional information	two nucleotide-binding sites with high and low affinity for GMP-CPP, one of the two sites constitutes the substrate site responsible for catalysis. The other site is the pseudosymmetric site, which exclusively serves as the binding site for YC-1 or BAY 41-2272	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
2'-dADP	mixture of competitive and noncompetitive inhibition, binding to the substrate site excludes the substrate GTP, whereas binding to the noncompetitive site has no effect on GTP binding, although the resulting complex is catalytically inactive. It binds to the high and low affinity sites with equivalent affinities. BAY 41-2272, which shares an analogous core structure to YC-1, fully inhibits 2'-dADP binding to the low affinity site, whereas the inhibition by YC-1 is incomplete	Bos taurus
adenosine 5'-beta,gamma-imido triphosphate	competitive inhibitor, reduces the Bmax for the binding of guanosine 5'-beta,gamma-methylene triphosphate, is tightly and selectively associated with the high affinity site	Bos taurus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	-	Bos taurus
Fe2+	-	Bos taurus
Mn2+	-	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
lung	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP	-	Bos taurus	3',5'-cyclic GMP + diphosphate	-	?
guanosine 5'-beta,gamma-methylene triphosphate	-	Bos taurus	?	-	?
additional information	2'-deoxy-3'-GMP and cGMP exhibit no detectable affinity for the enzyme	Bos taurus	?	-	?

Synonyms

Synonyms	Comment	Organism
sGC	-	Bos taurus
soluble guanylate cyclase	-	Bos taurus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
10	27	-	Bos taurus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0051	-	AMP-PNP	at 10°C	Bos taurus
0.015	-	AMP-PNP	at 27°C	Bos taurus

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Release 2023.1 (January 2023)