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Literature summary for 4.6.1.2 extracted from

  • Duda, T.; Sharma, R.K.
    ONE-GC membrane guanylate cyclase, a trimodal odorant signal transducer (2008), Biochem. Biophys. Res. Commun., 367, 440-445.
    View publication on PubMed
Search for more literature for 4.6.1.2

Activating Compound

Activating Compound Comment Organism Structure
additional information guanylin is totally ineffective in stimulating ONE-GC Rattus norvegicus
neurocalcin in the presence of the semimicromolar range of free Ca2+, neurocalcin binds to the catalytic module and stimulates ONE-GC, M880-L921 region of ONE-GC is the Ca2+-dependent neurocalcin binding and the transduction site of ONE-GC Rattus norvegicus
neurocalcin sigma dose-dependent stimulation in presence of the 0.01 mM Ca2+, 8fold over the basal value at ca. 0.002 mM Rattus norvegicus
uroguanylin stimulates ONE-GC through its external domain in a dose-dependent fashion Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
COS-7 cells transfected with the wild-type ONE-GC cDNA or its deletion mutants, ONE-GC mutants into pET30aLIC vector, expressed in Escherichia coli BL21-Codon-Plus-RIL Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information ONE-GCdeltaext mutant, deleted extracellular domain of ONE-GC, basal guanylate cyclase activity almost identical to that of wild-type, deletion of the extracellular domain has no effect on the tertiary structure of the protein, cannot be stimulated by uroguanylin. Tm bound mutant and soluble cat mutant, both have intrinsic catalytic activity, mutations have not effect on their tertiary structures Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
ONE-GC 880MGTTVEPEYFDQVTIYFSDIVG901 causes ca. 90% inhibition Rattus norvegicus
ONE-GC 900VGFTTISALSEPIEVVGFLNDL921 most effective inhibitor, causes ca. 95% inhibition Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on Ni-NTA affinity column Rattus norvegicus

Synonyms

Synonyms Comment Organism
GC-D
-
 Rattus norvegicus
ONE-GC
-
 Rattus norvegicus
ONE-GC membrane guanylate cyclase
-
 Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.06
-
-
 Rattus norvegicus 900VGFTTISALSEPIEVVGFLNDL921