Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NO | binds to the heme cofactor in the beta1 subunit, forming a five-coordinate NO complex that activates the enzyme several hundred-fold | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
N-terminal heme-binding regions of subunit beta1 from soluble guanylate cyclase are generated by subcloning specific constructs into the Escherichia coli expression vector pET-20b. The shortest region that is subcloned, has heme-bound, and is expressed well is beta1(1-194) | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | heme domain is localized to the N-terminal 194 residues of the beta1 subunit. Characterization of the minimum functional ligand-binding heme domain derived from soluble guanylate cyclase | Rattus norvegicus |