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Literature summary for 4.6.1.2 extracted from
- Sea urchin sperm guanylate cyclase. Purification and loss of cooperativity (1976), J. Biol. Chem., 251, 4071-4077.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
GTP | - |
Arbacia punctulata |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| particle-bound | - |
Arbacia punctulata | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Arbacia punctulata | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arbacia punctulata | - |
sea urchin | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Arbacia punctulata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spermatozoon | - |
Arbacia punctulata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12 | - |
- |
Arbacia punctulata |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 2 mM dithiothreitol, 25 mM triethanolamine, pH 7.4, 1 mM MnCl2, 20% glycerol, more than 1 month | Arbacia punctulata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | GTP in form of MnGTP2- | Arbacia punctulata | 3',5'-cyclic GMP + diphosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Arbacia punctulata |
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