Cloned (Comment) | Organism |
---|---|
expression of recombinant GST-fusion dominant negative mouse enzyme in Escherichia coli strain BL21, subcloning in Escherichia coli strain DH5alpha | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Mus musculus Swiss albino | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-fusion dominant negative mouse enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
heart | - |
Mus musculus | - |
kidney | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
lung | - |
Mus musculus | - |
additional information | RNase L mRNA is constitutively and differentially expressed in nine different mouse tissues, its level is maximal in immunological tissues (spleen, thymus and lungs), moderate in reproductive tissues (testis and prostate), and low in metabolic tissues (kidney, brain, liver and heart), semi-quantitative RT-PCR expression analysis, overview | Mus musculus | - |
prostate | - |
Mus musculus | - |
spleen | high expression level | Mus musculus | - |
testis | - |
Mus musculus | - |
thymus | - |
Mus musculus | - |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease L | - |
Mus musculus |
RNase L | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
physiological function | interferon-inducible 2',5'-oligoadenylate-dependent ribonuclease L is a uniquely regulated endoribonuclease, a key player in the innate antiviral immune defense mechanismsof mammalian cells induced by interferons. The enzyme might have more general cellular functions through protein-protein interactions in the spleen for immune response in mammals. Cellular enzyme-interacting proteins from spleen are fibronectin precursor, beta-actin, troponin I, myosin heavy chain 9 (non-muscle), growth-arrest specific protein 11, clathrin light chain B, a putative uncharacterized protein (Ricken cDNA 8030451F13) isoform (CRA d) and alanyl tRNA synthetase | Mus musculus |