Cloned (Comment) | Organism |
---|---|
using pGEM-T Easy vector | Danio rerio |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23700 | - |
sequence analysis | Danio rerio |
23700 | - |
x * 23700, calculated from sequence | Danio rerio |
25400 | - |
sequence analysis | Danio rerio |
27300 | - |
sequence analysis | Danio rerio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | B8XY54 | - |
- |
Danio rerio | B8XY55 | - |
- |
Danio rerio | B8XY56 | - |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration | Danio rerio |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adult | in all tissues | Danio rerio | - |
brain | - |
Danio rerio | - |
embryo | in all tissues | Danio rerio | - |
eye | - |
Danio rerio | - |
eye | low level of RNase Dre1 | Danio rerio | - |
gut | - |
Danio rerio | - |
heart | - |
Danio rerio | - |
liver | - |
Danio rerio | - |
additional information | RNase Dre1 is absent from liver | Danio rerio | - |
muscle | - |
Danio rerio | - |
ovary | - |
Danio rerio | - |
ovary | low level of RNase Dre1 | Danio rerio | - |
skin | - |
Danio rerio | - |
skin | low level of RNase Dre1 | Danio rerio | - |
testis | - |
Danio rerio | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA + H2O | - |
Danio rerio | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23700, calculated from sequence | Danio rerio |
? | x * 25400, calculated from sequence | Danio rerio |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease T2 | - |
Danio rerio |
RNase Dre1 | - |
Danio rerio |
RNase Dre2 | - |
Danio rerio |
RNase T2 | - |
Danio rerio |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
at an acidic pH stronger activities in the 20-30 kDa range that may correspond to RNase T2 enzymes | Danio rerio |
Organism | Comment | Expression |
---|---|---|
Danio rerio | RNase Dre2 expressed in all developmental stages and peaking at 72 h, signal is lower at 5 and 12 h | up |
Danio rerio | strong expression of RNase Dre1 during all developmental stages analyzed (2, 5, 12, 24, 72 h after fertilization), with a peak of expression at 72 hours | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the zebrafish genome contains two RNase T2 genes, RNase Dre1 and RNase Dre2. These genes are part of two phylogenetic clades, one conserved in all chordates (the RNase Dre2 clade), and another fish-specific (the RNase Dre1 clade). RNase T2 enzymes carry out a housekeeping function | Danio rerio |