Activating Compound | Comment | Organism | Structure |
---|---|---|---|
viral dsRNA | viral dsRNA provokes the synthesis of 2'-5' oligoadenylate, 2-5A, binding of 2-5A to ankyrin repeats of monomeric RNase L leads to RNase L dimerization and activation | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | it will be important, for proving advantageous in vaccines, to determine whether mutations in the RNA structure associated with the inhibition of RNAse L attenuate polyviruses or other group C enteroviruses in vivo | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
untagged recombinant human RNase L in a baculovirus vector is expressed in SF21 insect cells, into pcDNA3 vectors for stable transfection of HeLa cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R667A | dominant negative mutation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
poliovirus RNA | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA + H2O | Homo sapiens | RNase L cleaves RNAs predominantly after single-stranded UA and UU dinucleotides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using CL6B Blue Sepharose affinity resin, a MonoQ column | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | transfected with pcDNA3 vectors to establish a cell line overexpressing wild-type RNase L, with a dominant negative mutation, and control HeLa cells carrying the vector alone | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA + H2O | RNase L cleaves RNAs predominantly after single-stranded UA and UU dinucleotides | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
RNase L | - |
Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | RNA cleavage assay | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
RNA cleavage assay | Homo sapiens |