Cloned (Comment) | Organism |
---|---|
for overexpression in Escherichia coli BL21DE3 cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
wild-type RNase II is crystallized in two crystal forms, diffraction data are collected to 2.44 and 2.75 A resolution, the D209N mutant crystals diffract to 2.74 A | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D209N | catalytically inactive mutant, able to bind the substrate | Escherichia coli |
additional information | in addition the selenomethionine-derivatized D209N protein is produced | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
determined by SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a HisTrap-Chelating HP column, the His-tag is removed in some batches, using biotinylated thrombin | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease II | - |
Escherichia coli |
ribonuclease T2 | - |
Escherichia coli |
RNase II | - |
Escherichia coli |