Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.6.1.19 extracted from

  • McVey, C.E.; Amblar, M.; Barbas, A.; Cairrao, F.; Coelho, R.; Romao, C.; Arraiano, C.M.; Carrondo, M.A.; Frazao, C.
    Expression, purification, crystallization and preliminary diffraction data characterization of Escherichia coli ribonuclease II (RNase II) (2006), Acta Crystallogr. Sect. F, F62, 684-687.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
for overexpression in Escherichia coli BL21DE3 cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type RNase II is crystallized in two crystal forms, diffraction data are collected to 2.44 and 2.75 A resolution, the D209N mutant crystals diffract to 2.74 A Escherichia coli

Protein Variants

Protein Variants Comment Organism
D209N catalytically inactive mutant, able to bind the substrate Escherichia coli
additional information in addition the selenomethionine-derivatized D209N protein is produced Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
determined by SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a HisTrap-Chelating HP column, the His-tag is removed in some batches, using biotinylated thrombin Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
ribonuclease II
-
Escherichia coli
ribonuclease T2
-
Escherichia coli
RNase II
-
Escherichia coli