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Literature summary for 4.6.1.19 extracted from

  • Kawano, S.; Kakuta, Y.; Nakashima, T.; Kimura, M.
    Crystal structures of the Nicotiana glutinosa ribonuclease NT in complex with nucleoside monophosphates (2006), J. Biochem., 140, 375-381.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, expression plasmid pPIC9K, host strain GS115 Nicotiana glutinosa

Crystallization (Commentary)

Crystallization (Comment) Organism
determined in complex with either 5'-AMP, 5'-GMP, or 2'-UMP, 1.8 A resolution, data collections and refinement statistics shown, 5'-GMP and 5'-AMP bind both B1 and B2 sites, 2'-UMP predominantly binds B1 site in the crystallized complex, Trp42, Asn44, and Trp50 involved in base-interaction at the primary B1 site, Gln12, Tyr17, Ser78, Leu79, and Phe89 involved in base-recognition at the B2 subsite Nicotiana glutinosa

Organism

Organism UniProt Comment Textmining
Nicotiana glutinosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Nicotiana glutinosa

Source Tissue

Source Tissue Comment Organism Textmining
leaf infected with tobacco mosaic virus Nicotiana glutinosa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information induced upon tobacco mosaic virus infection, structure determined, substrate-binding sites studied with nucleoside monophosphates at concentrations between 1 and 10 mM followed by crystallization Nicotiana glutinosa ?
-
?

Synonyms

Synonyms Comment Organism
ribonuclease NT belongs to the RNase T2 family Nicotiana glutinosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information consists of seven helices and seven beta strands, determined by crystallization Nicotiana glutinosa