Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, expression plasmid pPIC9K, host strain GS115 | Nicotiana glutinosa |
Crystallization (Comment) | Organism |
---|---|
determined in complex with either 5'-AMP, 5'-GMP, or 2'-UMP, 1.8 A resolution, data collections and refinement statistics shown, 5'-GMP and 5'-AMP bind both B1 and B2 sites, 2'-UMP predominantly binds B1 site in the crystallized complex, Trp42, Asn44, and Trp50 involved in base-interaction at the primary B1 site, Gln12, Tyr17, Ser78, Leu79, and Phe89 involved in base-recognition at the B2 subsite | Nicotiana glutinosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana glutinosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Nicotiana glutinosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | infected with tobacco mosaic virus | Nicotiana glutinosa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | induced upon tobacco mosaic virus infection, structure determined, substrate-binding sites studied with nucleoside monophosphates at concentrations between 1 and 10 mM followed by crystallization | Nicotiana glutinosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease NT | belongs to the RNase T2 family | Nicotiana glutinosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | consists of seven helices and seven beta strands, determined by crystallization | Nicotiana glutinosa |