Any feedback?
Literature summary for 4.6.1.19 extracted from
- Purification and characterization of an extracellular, uracil specific ribonuclease from a Bizionia species isolated from the marine environment of the Sundarbans (2008), Microbiol. Res., 163, 31-38.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | isolate could be a novel renewable source of DNase-free RNase enzyme | Bacteroides sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Bacteroides sp. |  |
| Fe2+ | - |
Bacteroides sp. | |
| Hg2+ | - |
Bacteroides sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | improves the activity to some extent | Bacteroides sp. | |
| Ca2+ | improves the activity to some extent | Bacteroides sp. | |
| Mg2+ | enhances the activity by 1.5 times | Bacteroides sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
determined by SDS-PAGE | Bacteroides sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bacteroides sp. | RNase degrades uracil containing nucleic acids only | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteroides sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a CM-cellulose column, a DEAE-cellulose column and a Sephadex-G100 column | Bacteroides sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 22 | - |
purification step crude extract | Bacteroides sp. |
| 89 | - |
purification step acetone precipitation | Bacteroides sp. |
| 251 | - |
purification step CM-cellulose ion exchange | Bacteroides sp. |
| 426 | - |
purification step DEAE ion exchange | Bacteroides sp. |
| 630 | - |
purification step Sephadex gel filtration | Bacteroides sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | RNase degrades uracil containing nucleic acids only | Bacteroides sp. | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribonuclease | - |
Bacteroides sp. |
| RNase | - |
Bacteroides sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Bacteroides sp. |
| 37 | - |
activity assay | Bacteroides sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | - |
Bacteroides sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
activity assay | Bacteroides sp. |
| 6.5 | - |
- |
Bacteroides sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | enzyme retains more than half of its activity | Bacteroides sp. |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
