Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain R27-7C-1C | Rhizopus niveus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of enzyme mutants which possess designed substrate specificity, conversion of the base-nonspecific wild-type to base-specific mutants | Rhizopus niveus |
Q32D | site-directed mutagenesis, 26% activity compared to the wild-type enzyme | Rhizopus niveus |
Q32E | site-directed mutagenesis, 78% activity compared to the wild-type enzyme | Rhizopus niveus |
Q32F | site-directed mutagenesis, 9.3% activity compared to the wild-type enzyme | Rhizopus niveus |
Q32L | site-directed mutagenesis, 112% activity compared to the wild-type enzyme | Rhizopus niveus |
Q32N | site-directed mutagenesis, 21% activity compared to the wild-type enzyme | Rhizopus niveus |
Q32T | site-directed mutagenesis, 125% activity compared to the wild-type enzyme | Rhizopus niveus |
Q32V | site-directed mutagenesis, 39% activity compared to the wild-type enzyme | Rhizopus niveus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values for mutant enzymes with diverse dinucleotide substrates, overview | Rhizopus niveus | |
0.044 | - |
GpG | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
0.047 | - |
CpC | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
0.052 | - |
ApG | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
0.06 | - |
UpG | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
0.065 | - |
ApA | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
0.066 | - |
UpC | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
0.069 | - |
GpA | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
0.072 | - |
ApU | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
0.075 | - |
GpU | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
0.081 | - |
CpU | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
0.107 | - |
GpC | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
0.113 | - |
UpU | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
0.117 | - |
CpG | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
0.12 | - |
ApC | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
0.13 | - |
CpA | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
0.144 | - |
UpA | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizopus niveus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Saccharomyces cerevisiae | Rhizopus niveus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
RNA + H2O = an [RNA fragment]-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] | active site structure, Glu32 is an essential part of the B2-substrate recognition and binding site | Rhizopus niveus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
150 | - |
purified recombinant mutant Q32F | Rhizopus niveus |
330 | - |
purified recombinant mutant Q32N | Rhizopus niveus |
420 | - |
purified recombinant mutant Q32D | Rhizopus niveus |
620 | - |
purified recombinant mutant Q32V | Rhizopus niveus |
1250 | - |
purified recombinant mutant Q32E | Rhizopus niveus |
1600 | - |
purified recombinant wild-type enzyme | Rhizopus niveus |
1800 | - |
purified recombinant mutant Q32L | Rhizopus niveus |
2000 | - |
purified recombinant mutant Q32T | Rhizopus niveus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ApA + H2O | - |
Rhizopus niveus | AMP + adenosine | - |
? | |
ApC + H2O | - |
Rhizopus niveus | AMP + cytosine | - |
? | |
ApG + H2O | - |
Rhizopus niveus | AMP + guanosine | - |
? | |
ApU + H2O | - |
Rhizopus niveus | AMP + uridine | - |
? | |
CpA + H2O | - |
Rhizopus niveus | CMP + adenosine | - |
? | |
CpC + H2O | - |
Rhizopus niveus | CMP + cytidine | - |
? | |
CpG + H2O | - |
Rhizopus niveus | CMP + guanosine | - |
? | |
CpU + H2O | - |
Rhizopus niveus | 3'-CMP + uridine | - |
? | |
GpA + H2O | - |
Rhizopus niveus | GMP + adenosine | - |
? | |
GpC + H2O | - |
Rhizopus niveus | GMP + cytosine | - |
? | |
GpG + H2O | best substrate | Rhizopus niveus | GMP + guanosine | - |
? | |
GpU + H2O | - |
Rhizopus niveus | GMP + uridine | - |
? | |
additional information | subsite base specificities at the B1 and B2 site of wild-type and mutant enzymes, overview | Rhizopus niveus | ? | - |
? | |
RNA + H2O | from yeast | Rhizopus niveus | nucleoside 3'-phosphates and 3'-phosphooligonucleotides | via oligonucleotides or mononucleotides with 2',3'-cyclic phosphate at the 3'-side | ? | |
UpA + H2O | - |
Rhizopus niveus | UMP + adenosine | - |
? | |
UpC + H2O | - |
Rhizopus niveus | UMP + cytosine | - |
? | |
UpG + H2O | - |
Rhizopus niveus | UMP + guanosine | - |
? | |
UpU + H2O | - |
Rhizopus niveus | UMP + uridine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
base-nonspecific RNase | - |
Rhizopus niveus |
More | enzyme belongs to the RNase T2 family | Rhizopus niveus |
RNase Rh | - |
Rhizopus niveus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rhizopus niveus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Rhizopus niveus |