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Literature summary for 4.6.1.19 extracted from

  • Numata, T.; Kashiba, T.; Hino, M.; Funatsu, G.; Ishiguro, M.; Yamasaki, N.; Kimura, M.
    Expression and mutational analysis of amino acid residues involved in catalytic activity in a ribonuclease MC1 from the seeds of bitter gourd (2000), Biosci. Biotechnol. Biochem., 64, 603-605.
    View publication on PubMed
Search for more literature for 4.6.1.19

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of wild-type and mutant enzymes in Escherichia coli BL21(DE3) Momordica charantia

Protein Variants

Protein Variants Comment Organism
E84A site-directed mutagenesis, decreased kcat, 90% reduced activity compared to the wild-type enzyme Momordica charantia
H34A site-directed mutagenesis, active site residue, inactive mutant Momordica charantia
H83A site-directed mutagenesis, decreased kcat, 80% reduced activity compared to the wild-type enzyme Momordica charantia
H88A site-directed mutagenesis, active site residue, inactive mutant Momordica charantia
K87A site-directed mutagenesis, decreased kcat, 45% reduced activity compared to the wild-type enzyme Momordica charantia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.47
-
 CpU wild-type enzyme Momordica charantia
2.58
-
 CpU mutant K87A Momordica charantia
8.31
-
 CpU mutant H83A Momordica charantia
11.2
-
 CpU mutant E84A Momordica charantia

Organism

Organism UniProt Comment Textmining
Momordica charantia
-
 bitter gourd
-

Reaction

Reaction Comment Organism Reaction ID
RNA + H2O = an [RNA fragment]-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] residues His34, His 83, Glu84, Lys87, and His88 may be involved in catalysis Momordica charantia
a

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Momordica charantia
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CpU + H2O
-
 Momordica charantia ?
-
 ?
additional information transphosphorylation Momordica charantia ?
-
 ?
RNA + H2O from yeast Momordica charantia ?
-
 ?

Synonyms

Synonyms Comment Organism
More enzyme belongs to the ribonuclease T2 family Momordica charantia
ribonuclease MC1
-
 Momordica charantia
RNase MC1
-
 Momordica charantia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information wild-type and mutant enzymes Momordica charantia