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Literature summary for 4.6.1.19 extracted from
- Enzymatic properties of phenylalanine 101 mutant enzyme of ribonuclease Rh from Rhizopus niveus (2000), Biosci. Biotechnol. Biochem., 64, 2068-2074.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of mutant enzymes in Saccharomyces cerevisiae R27-7C-1C, secretion to culture medium | Rhizopus niveus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F101A | site-directed mutagenesis, below 1% activity compared to the wild-type enzyme | Rhizopus niveus |
| F101I | site-directed mutagenesis, 3.8% activity compared to the wild-type enzyme | Rhizopus niveus |
| F101K | site-directed mutagenesis, below 1% activity compared to the wild-type enzyme | Rhizopus niveus |
| F101L | site-directed mutagenesis, 20% activity compared to the wild-type enzyme | Rhizopus niveus |
| F101Q | site-directed mutagenesis, below 1% activity compared to the wild-type enzyme | Rhizopus niveus |
| F101W | site-directed mutagenesis, 7% activity compared to the wild-type enzyme | Rhizopus niveus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km values for mutant enzymes with diverse dinucleotide substrates, overview | Rhizopus niveus | |
| 0.045 | - |
GpG | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus |  |
| 0.049 | - |
CpC | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
| 0.052 | - |
ApG | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
| 0.063 | - |
UpG | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
| 0.065 | - |
ApA | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
| 0.068 | - |
UpC | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
| 0.072 | - |
GpA | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
| 0.076 | - |
CpU | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
| 0.078 | - |
ApU | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
| 0.079 | - |
GpU | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
| 0.11 | - |
GpC | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
| 0.11 | - |
CpG | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
| 0.13 | - |
ApC | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
| 0.13 | - |
CpA | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
| 0.13 | - |
UpU | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
| 0.14 | - |
UpA | wild-type enzyme, pH 5.0, 23°C | Rhizopus niveus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizopus niveus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant mutant enzymes from culture supernatant of transformed Saccharomyces cerevisiae | Rhizopus niveus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| RNA + H2O = an [RNA fragment]-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] | Phe101 is important for catalytic activity | Rhizopus niveus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.18 | - |
purified recombinant mutant F101K | Rhizopus niveus |
| 0.7 | - |
purified recombinant mutant F101R | Rhizopus niveus |
| 7.8 | - |
purified recombinant mutant F101A | Rhizopus niveus |
| 8.6 | - |
purified recombinant mutant F101Q | Rhizopus niveus |
| 60 | - |
purified recombinant mutant F101I | Rhizopus niveus |
| 110 | - |
purified recombinant mutant F101W | Rhizopus niveus |
| 340 | - |
purified recombinant mutant F101L | Rhizopus niveus |
| 1600 | - |
purified wild-type enzyme | Rhizopus niveus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ApA + H2O | - |
Rhizopus niveus | AMP + adenosine | - |
? | |
| ApC + H2O | - |
Rhizopus niveus | AMP + cytosine | - |
? | |
| ApG + H2O | - |
Rhizopus niveus | AMP + guanosine | - |
? | |
| ApU + H2O | - |
Rhizopus niveus | AMP + uridine | - |
? | |
| CpA + H2O | - |
Rhizopus niveus | CMP + adenosine | - |
? | |
| CpC + H2O | - |
Rhizopus niveus | CMP + cytidine | - |
? | |
| CpG + H2O | - |
Rhizopus niveus | CMP + guanosine | - |
? | |
| CpU + H2O | - |
Rhizopus niveus | 3'-CMP + uridine | - |
? | |
| GpA + H2O | - |
Rhizopus niveus | GMP + adenosine | - |
? | |
| GpC + H2O | - |
Rhizopus niveus | GMP + cytosine | - |
? | |
| GpG + H2O | best substrate | Rhizopus niveus | GMP + guanosine | - |
? | |
| GpU + H2O | - |
Rhizopus niveus | GMP + uridine | - |
? | |
| additional information | subsite base specificities at the B1 and B2 site of wild-type and mutant enzymes, overview | Rhizopus niveus | ? | - |
? | |
| RNA + H2O | - |
Rhizopus niveus | ? | - |
? | |
| UpA + H2O | - |
Rhizopus niveus | UMP + adenosine | - |
? | |
| UpC + H2O | - |
Rhizopus niveus | UMP + cytosine | - |
? | |
| UpG + H2O | - |
Rhizopus niveus | UMP + guanosine | - |
? | |
| UpU + H2O | - |
Rhizopus niveus | UMP + uridine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| base-nonspecific RNase Rh | - |
Rhizopus niveus |
| More | enzyme belongs to the ribonuclease T2 family | Rhizopus niveus |
| RNase Rh | - |
Rhizopus niveus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay temperatures depending on substrate | Rhizopus niveus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Rhizopus niveus |
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