Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-AMP | - |
Aspergillus oryzae | |
2'-CMP | - |
Aspergillus oryzae | |
2'-GMP | - |
Aspergillus oryzae | |
3'-AMP | - |
Aspergillus oryzae | |
AgNO3 | - |
Aspergillus oryzae | |
Cu2+ | - |
Aspergillus oryzae | |
Fe2+ | - |
Aspergillus oryzae | |
Hg2+ | - |
Aspergillus oryzae | |
MgCl2 | slight | Aspergillus oryzae | |
Mn2+ | - |
Aspergillus oryzae | |
Na2S | - |
Aspergillus oryzae | |
NaCl | - |
Aspergillus oryzae | |
Zn2+ | - |
Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
2',3'-cAMP | - |
Aspergillus oryzae | |
0.4 | - |
2',3'-cCMP | - |
Aspergillus oryzae | |
6.1 | - |
2',3'-cUMP | - |
Aspergillus oryzae | |
6.5 | - |
2',3'-cGMP | - |
Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36200 | - |
amino acid analysis | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 12-15% carbohydrate, RNase T2-A: mannose, glucose, RNase T2-B: mannose galactose | Aspergillus oryzae |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus oryzae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
RNA + H2O = an [RNA fragment]-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] | two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates: first step reversible cleavage of phosphodiester bond between 3'-adenylic acid group and 5'-hydroxyl group at adjacent nucleotides in RNA chain with formation of nucleoside 2',3'-cyclic phosphates and oligonucleotides with 2',3'-cyclic phosphate at 3'-terminal, second step: hydrolysis of terminal cyclic phosphate groups with formation of corresponding 3'-phosphates | Aspergillus oryzae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | Takadiastase powder | Aspergillus oryzae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-cAMP + H2O | - |
Aspergillus oryzae | ? | - |
? | |
2',3'-cCMP + H2O | - |
Aspergillus oryzae | ? | - |
? | |
2',3'-cGMP + H2O | - |
Aspergillus oryzae | ? | - |
? | |
2',3'-cUMP + H2O | - |
Aspergillus oryzae | ? | - |
? | |
RNA + H2O | two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates, first step reversible cleavage of phosphodiester bond between 3'-adenylic acid group and 5'-hydroxyl group at adjacent nucleotides in RNA chain with formation of nucleoside 2',3'-cyclic phosphates and oligonucleotides with 2',3'-cyclic phosphate at 3'-terminal, second step: hydrolysis of terminal cyclic phosphate groups with formation of corresponding 3'-phosphates, no base specificity | Aspergillus oryzae | 3'-phosphomononucleotides | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
pH 6.0, 5 min | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
RNA or homopolymer digestion | Aspergillus oryzae |
6 | 6.3 | hydrolysis of various nucleoside 2',3'-cyclic phosphates, degradation of 3'-nucleotide benzyl ester | Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
80°C, 5 min | Aspergillus oryzae |