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Literature summary for 4.6.1.19 extracted from

  • Uchida, T.; Egami, F.
    Microbial ribonucleases with special reference to RNases T1, T2, N1, and U2 (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 205-250.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2'-AMP
-
Aspergillus oryzae
2'-CMP
-
Aspergillus oryzae
2'-GMP
-
Aspergillus oryzae
3'-AMP
-
Aspergillus oryzae
AgNO3
-
Aspergillus oryzae
Cu2+
-
Aspergillus oryzae
Fe2+
-
Aspergillus oryzae
Hg2+
-
Aspergillus oryzae
MgCl2 slight Aspergillus oryzae
Mn2+
-
Aspergillus oryzae
Na2S
-
Aspergillus oryzae
NaCl
-
Aspergillus oryzae
Zn2+
-
Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
2',3'-cAMP
-
Aspergillus oryzae
0.4
-
2',3'-cCMP
-
Aspergillus oryzae
6.1
-
2',3'-cUMP
-
Aspergillus oryzae
6.5
-
2',3'-cGMP
-
Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36200
-
amino acid analysis Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 12-15% carbohydrate, RNase T2-A: mannose, glucose, RNase T2-B: mannose galactose Aspergillus oryzae

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus oryzae

Reaction

Reaction Comment Organism Reaction ID
RNA + H2O = an [RNA fragment]-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates: first step reversible cleavage of phosphodiester bond between 3'-adenylic acid group and 5'-hydroxyl group at adjacent nucleotides in RNA chain with formation of nucleoside 2',3'-cyclic phosphates and oligonucleotides with 2',3'-cyclic phosphate at 3'-terminal, second step: hydrolysis of terminal cyclic phosphate groups with formation of corresponding 3'-phosphates Aspergillus oryzae

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation Takadiastase powder Aspergillus oryzae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-cAMP + H2O
-
Aspergillus oryzae ?
-
?
2',3'-cCMP + H2O
-
Aspergillus oryzae ?
-
?
2',3'-cGMP + H2O
-
Aspergillus oryzae ?
-
?
2',3'-cUMP + H2O
-
Aspergillus oryzae ?
-
?
RNA + H2O two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates, first step reversible cleavage of phosphodiester bond between 3'-adenylic acid group and 5'-hydroxyl group at adjacent nucleotides in RNA chain with formation of nucleoside 2',3'-cyclic phosphates and oligonucleotides with 2',3'-cyclic phosphate at 3'-terminal, second step: hydrolysis of terminal cyclic phosphate groups with formation of corresponding 3'-phosphates, no base specificity Aspergillus oryzae 3'-phosphomononucleotides
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
pH 6.0, 5 min Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
RNA or homopolymer digestion Aspergillus oryzae
6 6.3 hydrolysis of various nucleoside 2',3'-cyclic phosphates, degradation of 3'-nucleotide benzyl ester Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
80°C, 5 min Aspergillus oryzae