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Literature summary for 4.6.1.19 extracted from

  • Imada, A.; Hunt, J.W.; van de Sande, H.; Sinskey, A.J.; Tannenbaum, S.R.
    Purification and properties of an intracellular ribonuclease from Candida lipolytica (1975), Biochim. Biophys. Acta, 395, 490-500.
    View publication on PubMed
Search for more literature for 4.6.1.19

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Yarrowia lipolytica
Zn2+
-
 Yarrowia lipolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 gel filtration Yarrowia lipolytica

Organism

Organism UniProt Comment Textmining
Yarrowia lipolytica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Yarrowia lipolytica

Reaction

Reaction Comment Organism Reaction ID
RNA + H2O = an [RNA fragment]-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] similar enzyme with possilbly exonucleolytic mechanism Yarrowia lipolytica
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Yarrowia lipolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: DNA Yarrowia lipolytica ?
-
 ?
RNA + H2O tRNA Yarrowia lipolytica 3'-phosphomononucleotides
-
 ?
RNA + H2O rRNA Yarrowia lipolytica 3'-phosphomononucleotides
-
 ?
RNA + H2O polyguanylic acid and duplexes of synthetic homopolymers less sensitive Yarrowia lipolytica 3'-phosphomononucleotides
-
 ?
RNA + H2O polyuridylic acid Yarrowia lipolytica 3'-phosphomononucleotides
-
 ?
RNA + H2O polyadenylic acid Yarrowia lipolytica 3'-phosphomononucleotides
-
 ?
RNA + H2O preference for pC-purine bonds Yarrowia lipolytica 3'-phosphomononucleotides
-
 ?
RNA + H2O polycytidylic acid Yarrowia lipolytica 3'-phosphomononucleotides
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Yarrowia lipolytica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 neutral pH, up to 50°C Yarrowia lipolytica
64
-
 30 min, pH 4.2: 95% inactivation, pH 6.6: 49% inactivation, pH 10.0: 64% inactivation Yarrowia lipolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Yarrowia lipolytica