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Literature summary for 4.6.1.19 extracted from
- Overexpression, purification, and properties of Escherichia coli ribonuclease II (1996), J. Biol. Chem., 271, 1048-1053.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| substrate and product stalilizes | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | enhances activity | Escherichia coli |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 223500 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNA + H2O | hydrolyzes in 3'- to 5'-direction, hydrolyzes single stranded RNA | Escherichia coli | 3'-phosphomononucleotides | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
RNA | - |
Escherichia coli | |
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