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Literature summary for 4.6.1.18 extracted from
- A ribonuclease inhibitor resistant dimer of human pancreatic ribonuclease displays specific antitumor activity (2018), Int. J. Biol. Macromol., 107, 1965-1970 .
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | a recombinant ribonuclease-resistant dimeric variant of human pancreatic ribonuclease is generated which is specifically toxic to cancer cells. Combining dimerization of pancreatic ribonuclease-inhibitor-resistance results in providing potent anti-tumor activity to human pancreatic ribonuclease. The cytotoxic variants of human pancreatic ribonuclease will be useful in designing protein therapeutics with low immunogenicity | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain BL21 Codon Plus (x04DE3)-RIL | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K7A/N71A/E111A | ribonuclease inhibitor-resistant cytotoxic variant | Homo sapiens |
| additional information | engineering of human pancreatic ribonuclease to develop two dimeric ribonuclease inhibitor-resistant molecules having anti-tumor activity. By incorporating two cysteines in human pancreatic ribonuclease and HPR-KNE (containing K7A/N71A/E111A mutations), a disulfide-linked dimeric pancreatic ribonuclease is generated, and a dimer of HPR-KNE, termed as HPR-D and HPR-KNE-D respectively. HPR-KNE-D is resistant towards inhibition by ribonuclease inhibitors, and is found to be highly toxic to a variety of cells. On J774A.1cells HPR-KNE-D is more than 375fold more cytotoxic than wild-type enzyme, and 15fold more toxic than HPR-D. Further,on U373 cells HPR-KNE-D is a more than 65fold more cytotoxic than HPR, and 9fold more toxic than HPR-D | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P07998 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Homo sapiens | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HPR | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme lacks cytotoxic activity as it is inactivated by intracellular cytosolic ribonuclease inhibitor | Homo sapiens |
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