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Literature summary for 4.6.1.18 extracted from

  • Fiorini, C.; Gotte, G.; Donnarumma, F.; Picone, D.; Donadelli, M.
    Bovine seminal ribonuclease triggers Beclin1-mediated autophagic cell death in pancreatic cancer cells (2014), Biochim. Biophys. Acta, 1843, 976-984.
    View publication on PubMed
Search for more literature for 4.6.1.18

Protein Variants

Protein Variants Comment Organism
G38K the mutant is more basic and interacts more strongly with the acidic membrane of cancer cells compared to the wild-type enzyme Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
RNase inhibitor RNase A, like most monomeric RNases, is strongly bound and inactivated in mammalian cells by the RNase inhibitor Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-
semen
-
 Bos taurus
-

Subunits

Subunits Comment Organism
dimer constituted by two identical subunits covalently bound through two antiparallel disulfide bridges Bos taurus
monomer
-
 Bos taurus
More native RNase A can be induced to form various N- or C-terminal domain-swapped dimers, trimers, and larger oligomers, all reconstituting the active site and augmenting the enzymatic activity against dsRNA with respect to the native monomer. RNase A oligomers can also acquire cytotoxic activity both in vitro and in vivo Bos taurus
oligomer the enzyme can oligomerize through the 3D domain swapping of both N- and C-termini, and its multimers is enzymatically and biologically more active than the native dimer Bos taurus

Synonyms

Synonyms Comment Organism
bovine seminal RNase
-
 Bos taurus
BS-RNase
-
 Bos taurus
RNase A
-
 Bos taurus
Seminal RNase
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Bos taurus

General Information

General Information Comment Organism
evolution the enzyme belongs to the pancreatic-type secretory ribonuclease superfamily Bos taurus
evolution the enzyme belongs to the pancreatic-type secretory ribonuclease superfamily as a unique natively dimeric member Bos taurus
physiological function the enzyme has almost no anti-tumoral property in pancreatic adenocarcinoma cell lines and in nontumorigenic cells as normal control, and is largely ineffective as anti-proliferative and pro-apoptotic agent Bos taurus
physiological function the enzyme has good anti-tumoral property in pancreatic adenocarcinoma cell lines and in nontumorigenic cells as normal control, it stimulates a strong anti-proliferative and pro-apoptotic effect in cancer cells. The enzyme triggers Beclin1-mediated autophagic cancer cell death, providing evidence that high proliferation rate of cancer cells may render them more susceptible to autophagy by treatment with the enzyme Bos taurus