Literature summary for 4.6.1.18 extracted from

Doucet, N.; Jayasundera, T.; Simonovic, M.; Loria, J.
The crystal structure of ribonuclease a in complex with thymidine-3'-monophosphate provides further insight into ligand binding (2010), Proteins, 78, 2459-2468.

Crystallization (Commentary)

Crystallization (Comment)	Organism
ribonuclease A in complex with thymidine 3'-monophosphate, hanging drop vapor diffusion method, 0.002 ml of 80 mg/ml protein in 20% ethanol and 20% acetic acid at pH 5.5, is mixed with 0.004 ml of 3'-TMP dissolved in a mother liquor solution of 20% ammonium sulfate and 2 M sodium chloride at pH 5.5, room temperature, 1 week, X-ray diffraction structure determination and analysis at 1.55 A resolution, molecular replacement, modelling	Bos taurus

Crystallization (Comment)

Organism

ribonuclease A in complex with thymidine 3'-monophosphate, hanging drop vapor diffusion method, 0.002 ml of 80 mg/ml protein in 20% ethanol and 20% acetic acid at pH 5.5, is mixed with 0.004 ml of 3'-TMP dissolved in a mother liquor solution of 20% ammonium sulfate and 2 M sodium chloride at pH 5.5, room temperature, 1 week, X-ray diffraction structure determination and analysis at 1.55 A resolution, molecular replacement, modelling

Bos taurus

Inhibitors

Inhibitors	Comment	Organism
3'-CMP	natural product inhibitor, NMR binding analysis, overview	Bos taurus
3'-TMP	a competitive inhibitor analogue of the 3'-CMP and 3'-UMP natural product inhibitors, the enzyme shows very high affinty and strong binding with 3'-TMP. Binding of 3'-TMP is very similar to other natural and nonnatural pyrimidine ligands, so single nucleotide affinity is independent of the presence or absence of a 2'-hydroxyl on the ribose moiety of pyrimidines	Bos taurus
3'-UMP	natural product inhibitor, NMR binding analysis, overview	Bos taurus
additional information	enzyme-inhibitor binding and interaction analysis, kinetics, overview	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P61823	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Bos taurus	-
pancreas	-	Bos taurus	-

Synonyms

Synonyms	Comment	Organism
pancreatic ribonuclease A	-	Bos taurus
RNase A	-	Bos taurus

General Information

General Information	Comment	Organism
additional information	very subtle structural, chemical, and potentially motional variations contribute to ligand discrimination in the enzyme	Bos taurus