Literature summary for 4.6.1.18 extracted from

Vila, R.; Benito, A.; Ribo, M.; Vilanova, M.
Mapping the stability clusters in bovine pancreatic ribonuclease A (2009), Biopolymers, 91, 1038-1047.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain BL21	Bos taurus

Protein Variants

Protein Variants	Comment	Organism
D53A	slight stabilization, increases the helix propensity of alpha-helix 3	Bos taurus
F120A	destabilization	Bos taurus
F46A	equivalent destabilization for F46A and F46L variants	Bos taurus
F46L	equivalent destabilization for F46A and F46L variants	Bos taurus
F8A	substitution of Phe8 results in a recombinant variant significantly destabilized	Bos taurus
F8L	substitution of Phe8 results in a recombinant variant significantly destabilized	Bos taurus
K7A	slight stabilization, increases the helix propensity of alpha-helix 1	Bos taurus
L35A	less destabilizing than M30A substitution	Bos taurus
L51A	shortening the side chain of the hydrophobic solvent-exposed residue Leu51 to Ala has almost no effect in the stability of ribonuclease A	Bos taurus
M13A	critical position for the ribonuclease A stability	Bos taurus
M30A	critical position for the ribonuclease A stability	Bos taurus
M79A	a minor role in the stabilization of the protein	Bos taurus
Q28A	promotes an increase in the helix propensity, from 0.99 in the wild-type to 1.5	Bos taurus
T87A	no effect in the protein stability	Bos taurus
V43A	substitution of the hydrophobic residue valin 43 by alanin results in an increase in the global stability of the ribonuclease A structure of 4.02 kJ/mol in free energy	Bos taurus
Y25A	critical position for the ribonuclease A stability	Bos taurus
Y25F	decreases the stability of the enzyme by 7.41 kJ/mol	Bos taurus
Y73A	a minor role in the stabilization of the protein	Bos taurus
Y97A	substitution of Tyr97 results in a variant significantly destabilized	Bos taurus
Y97L	substitution of Tyr97 results in a variant significantly destabilized	Bos taurus

General Stability

General Stability	Organism
calculation of thermodynamic parameters from thermally induced unfolding curves	Bos taurus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
13590	-	mutant enzyme Y73A, MALDI-TOF/TOF mass spectrometry	Bos taurus
13590	-	mutant enzyme Y97A, MALDI-TOF/TOF mass spectrometry	Bos taurus
13600	-	mutant enzyme Y25A, MALDI-TOF/TOF mass spectrometry	Bos taurus
13610	-	mutant enzyme F120A, MALDI-TOF/TOF mass spectrometry	Bos taurus
13610	-	mutant enzyme F46A, MALDI-TOF/TOF mass spectrometry	Bos taurus
13610	-	mutant enzyme F8A, MALDI-TOF/TOF mass spectrometry	Bos taurus
13620	-	mutant enzyme K7A, MALDI-TOF/TOF mass spectrometry	Bos taurus
13620	-	mutant enzyme M13A, MALDI-TOF/TOF mass spectrometry	Bos taurus
13620	-	mutant enzyme M30A, MALDI-TOF/TOF mass spectrometry	Bos taurus
13620	-	mutant enzyme Q28A, MALDI-TOF/TOF mass spectrometry	Bos taurus
13620	-	mutant enzyme T87A, MALDI-TOF/TOF mass spectrometry	Bos taurus
13630	-	mutant enzyme Y97L, MALDI-TOF/TOF mass spectrometry	Bos taurus
13640	-	mutant enzyme D53A, MALDI-TOF/TOF mass spectrometry	Bos taurus
13640	-	mutant enzyme L35A, MALDI-TOF/TOF mass spectrometry	Bos taurus
13640	-	mutant enzyme L51A, MALDI-TOF/TOF mass spectrometry	Bos taurus
13650	-	mutant enzyme F46L, MALDI-TOF/TOF mass spectrometry	Bos taurus
13650	-	mutant enzyme F8L, MALDI-TOF/TOF mass spectrometry	Bos taurus
13650	-	mutant enzyme M79A, MALDI-TOF/TOF mass spectrometry	Bos taurus
13660	-	mutant enzyme V43A, MALDI-TOF/TOF mass spectrometry	Bos taurus
13670	-	mutant enzyme Y25F, MALDI-TOF/TOF mass spectrometry	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P61823	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining

Synonyms

Synonyms	Comment	Organism
ribonuclease A	-	Bos taurus
RNase A	-	Bos taurus