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Literature summary for 4.6.1.18 extracted from

  • Shinomiya, K.; Kobayashi, H.; Inokuchi, N.; Kobayashi, K.; Oshima, H.; Kitanaka, S.; Yanagidaira, K.; Sasaki, H.; Muto, M.; Okano, M.; Ito, Y.
    New small-scale cross-axis coil planet centrifuge. Partition efficiency and application to purification of bullfrog ribonuclease (2007), J. Chromatogr. A, 1151, 91-98.
    View publication on PubMed

Application

Application Comment Organism
analysis studies on application to purify ribonuclease from eggs of Rana catesbeiana with an aqueous–aqueous polymer phase system by using a small-scale cross-axis coil planet centrifuge Lithobates catesbeianus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA + H2O Lithobates catesbeianus
-
3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
?

Organism

Organism UniProt Comment Textmining
Lithobates catesbeianus P14626 bullfrog
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration of crude egg extracts, a two-phase polymer system composed of 12.5% PEG 1000 and 12.5% dibasic potassium phosphate added to crude extracts prior to further purification by small-scale cross-axis coil planet centrifuge Lithobates catesbeianus

Source Tissue

Source Tissue Comment Organism Textmining
egg
-
Lithobates catesbeianus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA + H2O
-
Lithobates catesbeianus 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
?
RNA + H2O studies on application to the purification of ribonuclease of Rana catesbeiana eggs using an aqueous-aqueous polymer phase system and a small-scale cross-axis coil planet centrifuge Lithobates catesbeianus 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
?

Synonyms

Synonyms Comment Organism
pancreatic ribonuclease
-
Lithobates catesbeianus
ribonuclease A
-
Lithobates catesbeianus