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Literature summary for 4.6.1.18 extracted from

  • Nitto, T.; Dyer, K.D.; Czapiga, M.; Rosenberg, H.F.
    Evolution and function of leukocyte RNase A ribonucleases of the avian species, Gallus gallus (2006), J. Biol. Chem., 281, 25622-25634.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Gallus gallus

Protein Variants

Protein Variants Comment Organism
H110A complete loss of activity Gallus gallus
additional information substitution of domain I of Gallus gallus rRNase A-1 onto the rRNase A-2 backbone significantly attenuats bactericidal activity. Taken further, substitution of either domain II or III of rRNase A-1 alone, or domains II and III together eliminates the bactericidal activity of RNase A-2 Gallus gallus
additional information substitution of the RNase A-1 backbone with either domain I, and more so, II, or III of Gallus gallus RNase A-2 confers bactericidal activity on RNase A-1 Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
ribonuclease inhibitor human placental ribonuclease inhibitor; human placental ribonuclease inhibitor Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
tRNA pH 7.0 Gallus gallus
0.0172
-
tRNA pH 7.0 Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15767
-
x * 15767, calculated Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Gallus gallus enzyme is both angiogenic and bactericidal. Domains II, amino acids 71-76, and III, amino acids 89-104, of RNase A-2 are both important for bactericidal activity ?
-
?

Organism

Organism UniProt Comment Textmining
Gallus gallus Q27J90 isoform RNase A-2
-
Gallus gallus Q27J91 isoform RNase A-1
-

Source Tissue

Source Tissue Comment Organism Textmining
bone marrow
-
Gallus gallus
-
granulocyte peripheral blood granulocyte Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is both angiogenic and bactericidal. Domains II, amino acids 71-76, and III, amino acids 89-104, of RNase A-2 are both important for bactericidal activity Gallus gallus ?
-
?
tRNA + H2O substrate yeast tRNA Gallus gallus ?
-
?

Subunits

Subunits Comment Organism
? x * 15767, calculated Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.056
-
tRNA pH 7.0 Gallus gallus
2.6
-
tRNA pH 7.0 Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Gallus gallus

pI Value

Organism Comment pI Value Maximum pI Value
Gallus gallus isoform RNase A-1
-
10.2
Gallus gallus isoform RNase A-2
-
11
Gallus gallus isoform RNase A-2, calculated
-
11