Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Gallus gallus |
Protein Variants | Comment | Organism |
---|---|---|
H110A | complete loss of activity | Gallus gallus |
additional information | substitution of domain I of Gallus gallus rRNase A-1 onto the rRNase A-2 backbone significantly attenuats bactericidal activity. Taken further, substitution of either domain II or III of rRNase A-1 alone, or domains II and III together eliminates the bactericidal activity of RNase A-2 | Gallus gallus |
additional information | substitution of the RNase A-1 backbone with either domain I, and more so, II, or III of Gallus gallus RNase A-2 confers bactericidal activity on RNase A-1 | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ribonuclease inhibitor | human placental ribonuclease inhibitor; human placental ribonuclease inhibitor | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
tRNA | pH 7.0 | Gallus gallus | |
0.0172 | - |
tRNA | pH 7.0 | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15767 | - |
x * 15767, calculated | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Gallus gallus | enzyme is both angiogenic and bactericidal. Domains II, amino acids 71-76, and III, amino acids 89-104, of RNase A-2 are both important for bactericidal activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | Q27J90 | isoform RNase A-2 | - |
Gallus gallus | Q27J91 | isoform RNase A-1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone marrow | - |
Gallus gallus | - |
granulocyte | peripheral blood granulocyte | Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme is both angiogenic and bactericidal. Domains II, amino acids 71-76, and III, amino acids 89-104, of RNase A-2 are both important for bactericidal activity | Gallus gallus | ? | - |
? | |
tRNA + H2O | substrate yeast tRNA | Gallus gallus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 15767, calculated | Gallus gallus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
tRNA | pH 7.0 | Gallus gallus | |
2.6 | - |
tRNA | pH 7.0 | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Gallus gallus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Gallus gallus | isoform RNase A-1 | - |
10.2 |
Gallus gallus | isoform RNase A-2 | - |
11 |
Gallus gallus | isoform RNase A-2, calculated | - |
11 |