Literature summary for 4.6.1.18 extracted from

Schulenburg, C.; Ardelt, B.; Ardelt, W.; Arnold, U.; Shogen, K.; Ulbrich-Hofmann, R.; Darzynkiewicz, Z.
The interdependence between catalytic activity, conformational stability, and cytotoxicity of onconase (2007), Cancer Biol. Ther., 6, 1233-1239.

Search for more literature for 4.6.1.18

Protein Variants

Protein Variants	Comment	Organism
C87A/C104A	66% of wild-type activity, reduced cytotoxic and cytostatic properties, IC50 values for K-562 cells 0.0046 mM	Lithobates pipiens
F28A	61% of wild-type activity, reduced cytotoxic and cytostatic properties, IC50 values for K-562 cells 0.0043 mM	Lithobates pipiens
F28T	60% of wild-type activity, reduced cytotoxic and cytostatic properties, IC50 values for K-562 cells 0.0037 mM	Lithobates pipiens
F36A	69% of wild-type activity, reduced cytotoxic and cytostatic properties, IC50 values for K-562 cells 0.0039 mM	Lithobates pipiens
F36Y	80% of wild-type activity, reduced cytotoxic and cytostatic properties, IC50 values for K-562 cells 0.0027 mM	Lithobates pipiens
Glp1E	41% of wild-type activity, reduced cytotoxic and cytostatic properties, IC50 values for K-562 cells 0.0099 mM	Lithobates pipiens
glp1P	44% of wild-type activity, reduced cytotoxic and cytostatic properties, IC50 values for K-562 cells 0.015 mM	Lithobates pipiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Lithobates pipiens	enzyme expressed in K-562 cell is cytotoxic and cytostatic, IC50 value 0.0009 mM	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Lithobates pipiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
embryo	-	Lithobates pipiens	-
oocyte	-	Lithobates pipiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	enzyme expressed in K-562 cell is cytotoxic and cytostatic, IC50 value 0.0009 mM	Lithobates pipiens	?	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
69.4	-	mutant C87A/C104A, melting temperature	Lithobates pipiens
69.6	-	mutant F36A, melting temperature	Lithobates pipiens
77.4	-	mutant F28T, melting temperature	Lithobates pipiens
78.1	-	mutant F28A, melting temperature	Lithobates pipiens
79.2	-	mutant F36Y, melting temperature	Lithobates pipiens
87	-	mutant Glp1E, melting temperature	Lithobates pipiens
87.7	-	mutant Glp1P, melting temperature	Lithobates pipiens
88.5	-	wild-type, melting temperature	Lithobates pipiens

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Release 2023.1 (January 2023)