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Literature summary for 4.6.1.18 extracted from
- Pressure- and temperature-induced unfolding studies: thermodynamics of core hydrophobicity and packing of ribonuclease A (2006), Biol. Chem., 387, 285-296.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| I106A | detailed study on thermodynamic parameters | Bos taurus |
| I107A | detailed study on thermodynamic parameters | Bos taurus |
| I81A | detailed study on thermodynamic parameters | Bos taurus |
| additional information | series of mutations in residues V54, V57, I106 and V108 most critical for stability. Detailed study on thermodynamic parameters | Bos taurus |
| V108A | detailed study on thermodynamic parameters | Bos taurus |
| V116A | detailed study on thermodynamic parameters | Bos taurus |
| V118A | detailed study on thermodynamic parameters | Bos taurus |
| V47A | detailed study on thermodynamic parameters | Bos taurus |
| V54A | detailed study on thermodynamic parameters | Bos taurus |
| V57A | detailed study on thermodynamic parameters | Bos taurus |
| V63A | detailed study on thermodynamic parameters | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | P61823 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Bos taurus | - |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
heat and pressure produce only slightly different energetic changes in the unfolded state of enzyme. Stability differences in mutants can be attributed to both hydrophobic interactions and packing density | Bos taurus |
| 43.1 | - |
melting temperature, mutant I106A | Bos taurus |
| 43.3 | - |
melting temperature, mutant V108A | Bos taurus |
| 45 | - |
melting temperature, mutant V47A | Bos taurus |
| 47.7 | - |
melting temperature, mutant I107A | Bos taurus |
| 47.7 | - |
melting temperature, mutant I81A | Bos taurus |
| 48.7 | - |
melting temperature, mutant V57A | Bos taurus |
| 49.8 | - |
melting temperature, mutant V54A | Bos taurus |
| 52.5 | - |
melting temperature, mutant V118A | Bos taurus |
| 54.8 | - |
melting temperature, mutant V116A | Bos taurus |
| 58 | - |
melting temperature, wild-type | Bos taurus |
| 58.1 | - |
melting temperature, mutant V63A | Bos taurus |
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Release 2023.1 (January 2023)
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