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Literature summary for 4.6.1.18 extracted from

  • Pouckova, P.; Morbio, M.; Vottariello, F.; Laurents, D.V.; Matousek, J.; Soucek, J.; Gotte, G.; Donadelli, M.; Costanzo, C.; Libonati, M.
    Cytotoxicity of polyspermine-ribonuclease A and polyspermine-dimeric ribonuclease A (2007), Bioconjug. Chem., 18, 1946-1955.
    View publication on PubMed
Search for more literature for 4.6.1.18

Application

Application Comment Organism
medicine cross-linking of enzyme or its covalently linked dimer to polyspermine using dimethyl suberimidate. The in vitro and in vivo cytotoxic activity of treated monomeric enzyme is not higher than that of free polyspermine, but dimeric suberimidate-treated enzyme proves to be a more efficinet antitumor agent both in vitro and in vivo Bos taurus

Protein Variants

Protein Variants Comment Organism
additional information cross-linking of enzyme or its covalently linked dimer to polyspermine using dimethyl suberimidate. The in vitro and in vivo cytotoxic activity of treated monomeric enzyme is not higher than that of free polyspermine, but dimeric suberimidate-treated enzyme proves to be a more efficient antitumor agent both in vitro and in vivo Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 Type XII-A, commercial preparation
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14
-
 substrate poly(A)poly(U), dimethyl suberimidate-treated, monomeric RNase A, pH 7.0, 25°C Bos taurus
1.55
-
 substrate poly(A)poly(U), native, monomeric RNase A, pH 7.0, 25°C Bos taurus
2.22
-
 substrate poly(A)poly(U), cross-linked RNase A dimer, pH 7.0, 25°C Bos taurus
12.15
-
 substrate poly(A)poly(U), domain-swapped RNase A C-dimer pH 7.0, 25°C Bos taurus
12.67
-
 substrate yeast RNA, cross-linked RNase A dimer, pH 7.0, 25°C Bos taurus
13.33
-
 substrate yeast RNA, cross-linked RNase A dimer, treated with dimethyl suberimidate, pH 7.0, 25°C Bos taurus
25.21
-
 substrate poly(A)poly(U), cross-linked RNase A dimer, treated with dimethyl suberimidate, pH 7.0, 25°C Bos taurus
49.33
-
 substrate yeast RNA, dimethyl suberimidate-treated, monomeric RNase A, pH 7.0, 25°C Bos taurus
53.46
-
 substrate yeast RNA, domain-swapped RNase A C-dimer pH 7.0, 25°C Bos taurus
84.55
-
 substrate yeast RNA, native, monomeric RNase A, pH 7.0, 25°C Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A)poly(U) + H2O
-
 Bos taurus ?
-
 ?
RNA + H2O substrate yeast RNA Bos taurus 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?