Crystallization (Comment) | Organism |
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determination of solution structure of mutant F31A by NMR spectroscopy reveals the change in orientation of the W23 side chain, which in the wild type is completely exposed to the solvent, whereas in the mutant is largely buried in the aromatic cluster. Mutation leads to strong distortion in the alpha-sheets with loss in several hydrogen bonds, and increased flexibility of some stretches of the backbone | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
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F31A | loss in thermo and piezostabilities by at least 27 K and 10 kbar. Determination of solution structure by NMR spectroscopy reveals the change in orientation of the W23 side chain, which in the wild type is completely exposed to the solvent, whereas in the mutant is largely buried in the aromatic cluster. Mutation leads to strong distortion in the alpha-sheets with loss in several hydrogen bonds, and increased flexibility of some stretches of the backbone | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
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Saccharolobus solfataricus | P61991 | - |
- |