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Literature summary for 4.6.1.18 extracted from

  • Dey, P.; Islam, A.; Ahmad, F.; Batra, J.K.
    Role of unique basic residues of human pancreatic ribonuclease in its catalysis and structural stability (2007), Biochem. Biophys. Res. Commun., 360, 809-814.
    View publication on PubMed
Search for more literature for 4.6.1.18

Protein Variants

Protein Variants Comment Organism
K62A full catalytic activity, reduced protein stability and DNA unwinding activity Homo sapiens
K6A reduced catalytic activity on both ssRNA and dsRNA Homo sapiens
K74A altered seconday structure, reduced protein stability and DNA unwinding activity, reduced catalytic activity on both ssRNA and dsRNA Homo sapiens
R32A full catalytic activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 poly(A)poly(U) mutant K74A, pH 7.5, 37°C Homo sapiens
0.052
-
 poly(A)poly(U) mutant K62A, pH 7.5, 37°C Homo sapiens
0.063
-
 poly(A)poly(U) wild-type, pH 7.5, 37°C Homo sapiens
0.09
-
 poly(A)poly(U) mutant K6A, pH 7.5, 37°C Homo sapiens
0.1
-
 poly(C) wild-type, pH 7.5, 37°C Homo sapiens
0.16
-
 poly(A)poly(U) mutant R32A, pH 7.5, 37°C Homo sapiens
0.21
-
 poly(C) mutant R32A, pH 7.5, 37°C Homo sapiens
0.3
-
 poly(C) mutant K74A, pH 7.5, 37°C Homo sapiens
0.37
-
 poly(C) mutant K62A, pH 7.5, 37°C Homo sapiens
0.4
-
 poly(C) mutant K6A, pH 7.5, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A)poly(U) + H2O
-
 Homo sapiens ?
-
 ?
poly(C) + H2O
-
 Homo sapiens 3'-CMP + 3'-phosphooligonucleotides
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
32.1
-
 mutant K74A, first melting temperature Homo sapiens
51.8
-
 mutant K74A, second melting temperature Homo sapiens
53.2
-
 mutant K62A, melting temperature Homo sapiens
54.5
-
 mutant K6A, melting temperature Homo sapiens
54.8
-
 wild-type, melting temperature Homo sapiens
55
-
 mutant R32A, melting temperature Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0002
-
 poly(A)poly(U) mutant K74A, pH 7.5, 37°C Homo sapiens
0.0012
-
 poly(A)poly(U) mutant K6A, pH 7.5, 37°C Homo sapiens
0.0019
-
 poly(A)poly(U) wild-type, pH 7.5, 37°C Homo sapiens
0.0026
-
 poly(A)poly(U) mutant K62A, pH 7.5, 37°C Homo sapiens
0.0065
-
 poly(A)poly(U) mutant R32A, pH 7.5, 37°C Homo sapiens
0.11
-
 poly(C) mutant K74A, pH 7.5, 37°C Homo sapiens
0.192
-
 poly(C) mutant K6A, pH 7.5, 37°C Homo sapiens
0.242
-
 poly(C) wild-type, pH 7.5, 37°C Homo sapiens
0.57
-
 poly(C) mutant R32A, pH 7.5, 37°C Homo sapiens
0.83
-
 poly(C) mutant K62A, pH 7.5, 37°C Homo sapiens