Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D121A | Km-value for poly(C) is 2.4fold higher than wild-type value, Km-value for yeast tRNA is 1.25fold higher than wild-type value, turnover-number for poly(C) is 16.5fold lower than wild-type value, turnover-number for yeast tRNA is 1.3fold lower than wild-type value. ASp121 is crucial for the catalytic activity and may be involved in the depolymerization activity of the enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
poly(C) | pH 7.5, 37°C, wild-type enzyme | Homo sapiens | |
4 | - |
tRNA | pH 7.5, 37°C, wild-type enzyme | Homo sapiens | |
4 | - |
poly(C) | pH 7.5, 37°C, mutant enzyme D121A | Homo sapiens | |
5 | - |
tRNA | pH 7.5, 37°C, mutant enzyme D121A | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly (C) + H2O | - |
Homo sapiens | 3'-CMP + 3'-phosphooligonucleotides | - |
? | |
tRNA + H2O | tRNA from yeast | Homo sapiens | 3'-phosphomononucleotides + 3'-phosphooligonucleotides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HPR | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens |