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Literature summary for 4.6.1.18 extracted from

  • Chatani, E.; Tanimizu, N.; Ueno, H.; Hayashi, R.
    Structural and functional changes in bovine pancreatic ribonuclease A by the replacement of Phe120 with other hydrophobic residues (2001), J. Biochem., 129, 917-922.
    View publication on PubMed
Search for more literature for 4.6.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bos taurus

Protein Variants

Protein Variants Comment Organism
F120A with less thermal stability than the wild type enzyme Bos taurus
F120G with less thermal stability than the wild type enzyme Bos taurus
F120L with less thermal stability than the wild type enzyme Bos taurus
F120W with less thermal stability than the wild type enzyme Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
phosphate
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.62
-
 cytidine-2',3'-cyclic monophosphate wild type enzyme, pH 5.5, 25°C Bos taurus
0.67
-
 cytidinyl-3',5'-adenosine wild type enzyme, pH 5.5, 25°C Bos taurus
1.7
-
 cytidine-2',3'-cyclic monophosphate F120L mutant, pH 5.5, 25°C Bos taurus
3.5
-
 cytidinyl-3',5'-adenosine F120L mutant, pH 5.5, 25°C Bos taurus
4
-
 cytidine-2',3'-cyclic monophosphate F120W mutant, pH 5.5, 25°C Bos taurus
7.3
-
 cytidinyl-3',5'-adenosine F120W mutant, pH 5.5, 25°C Bos taurus
7.9
-
 cytidine-2',3'-cyclic monophosphate F120A mutant, pH 5.5, 25°C Bos taurus
13
-
 cytidinyl-3',5'-adenosine F120A mutant, pH 5.5, 25°C Bos taurus
13
-
 cytidine-2',3'-cyclic monophosphate F120G mutant, pH 5.5, 25°C Bos taurus
22
-
 cytidinyl-3',5'-adenosine F120G mutant, pH 5.5, 25°C Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 bovine
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytidine-2',3'-cyclic monophosphate + H2O
-
 Bos taurus 3'-CMP
-
 ?
cytidinyl-3',5'-adenosine + H2O CpA Bos taurus adenosine + 3'-CMP
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 mutant enzymes are less stable than the wild type enzyme Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.5
-
 phosphate F120G mutant, pH 5.5, 25°C Bos taurus
6.4
-
 phosphate wild type enzyme, pH 5.5, 25°C Bos taurus
10
-
 phosphate F120A mutant, pH 5.5, 25°C Bos taurus
10
-
 phosphate F120L mutant, pH 5.5, 25°C Bos taurus
11
-
 phosphate F120W mutant, pH 5.5, 25°C Bos taurus