Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
F120A | with less thermal stability than the wild type enzyme | Bos taurus |
F120G | with less thermal stability than the wild type enzyme | Bos taurus |
F120L | with less thermal stability than the wild type enzyme | Bos taurus |
F120W | with less thermal stability than the wild type enzyme | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.62 | - |
cytidine-2',3'-cyclic monophosphate | wild type enzyme, pH 5.5, 25°C | Bos taurus | |
0.67 | - |
cytidinyl-3',5'-adenosine | wild type enzyme, pH 5.5, 25°C | Bos taurus | |
1.7 | - |
cytidine-2',3'-cyclic monophosphate | F120L mutant, pH 5.5, 25°C | Bos taurus | |
3.5 | - |
cytidinyl-3',5'-adenosine | F120L mutant, pH 5.5, 25°C | Bos taurus | |
4 | - |
cytidine-2',3'-cyclic monophosphate | F120W mutant, pH 5.5, 25°C | Bos taurus | |
7.3 | - |
cytidinyl-3',5'-adenosine | F120W mutant, pH 5.5, 25°C | Bos taurus | |
7.9 | - |
cytidine-2',3'-cyclic monophosphate | F120A mutant, pH 5.5, 25°C | Bos taurus | |
13 | - |
cytidinyl-3',5'-adenosine | F120A mutant, pH 5.5, 25°C | Bos taurus | |
13 | - |
cytidine-2',3'-cyclic monophosphate | F120G mutant, pH 5.5, 25°C | Bos taurus | |
22 | - |
cytidinyl-3',5'-adenosine | F120G mutant, pH 5.5, 25°C | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
bovine | - |
Purification (Comment) | Organism |
---|---|
gel filtration | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytidine-2',3'-cyclic monophosphate + H2O | - |
Bos taurus | 3'-CMP | - |
? | |
cytidinyl-3',5'-adenosine + H2O | CpA | Bos taurus | adenosine + 3'-CMP | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
mutant enzymes are less stable than the wild type enzyme | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
phosphate | F120G mutant, pH 5.5, 25°C | Bos taurus | |
6.4 | - |
phosphate | wild type enzyme, pH 5.5, 25°C | Bos taurus | |
10 | - |
phosphate | F120A mutant, pH 5.5, 25°C | Bos taurus | |
10 | - |
phosphate | F120L mutant, pH 5.5, 25°C | Bos taurus | |
11 | - |
phosphate | F120W mutant, pH 5.5, 25°C | Bos taurus |