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Literature summary for 4.6.1.18 extracted from
- Expression of soluble bovine pancreatic ribonuclease A in Pichia pastoris and its purification and characterization (2000), Biosci. Biotechnol. Biochem., 64, 2437-2444.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Bos taurus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N34A | unglycosylated mutant | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
cytidine-2',3'-cyclic monophosphate | type I and II isoenzymes, almost identical values to those of commercial enzyme, pH 5.5, 25°C | Bos taurus |  |
| additional information | - |
CpA | type I and II isoenzymes, almost identical values to those of commercial enzyme, pH 5.5, 25°C | Bos taurus | |
| 0.45 | - |
cytidine-2',3'-cyclic monophosphate | pH 5.5, 25ºC, comercial enzyme | Bos taurus | |
| 0.46 | - |
cytidine-2',3'-cyclic monophosphate | pH 5.5, 25ºC, type I enzyme | Bos taurus | |
| 0.5 | - |
cytidinyl-3',5'-adenosine | pH 5.5, 25ºC, comercial enzyme | Bos taurus | |
| 0.52 | - |
cytidinyl-3',5'-adenosine | pH 5.5, 25ºC, type I enzyme | Bos taurus | |
| 0.58 | - |
cytidine-2',3'-cyclic monophosphate | pH 5.5, 25ºC, N34A mutant | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13640 | - |
N34A mutant, mass spectrometry | Bos taurus |
| 13680 | - |
type I isoenzyme, mass spectrometry | Bos taurus |
| 13850 | 15310 | type II isoenzyme, multiple mass peaks, mass spectrometry | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
bovine | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | type II isoenzyme, O-linked glycoform | Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cation exchange chromatography | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
type I isoform, 100% specific activity relative to commercial RNase A. Type II isoform, 53% specific activity relative to commercial enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CpA + H2O | - |
Bos taurus | adenosine + 3'-CMP | - |
? | |
| cytidine-2',3'-cyclic monophosphate + H2O | hydrolysis reaction | Bos taurus | 3'-CMP | - |
? | |
| cytidinyl-3',5'-adenosine + H2O | CpA, transphosphorylation reaction | Bos taurus | adenosine + 3'-CMP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| type I RNase A | isoform with the same molecular mass as that of a commercial RNase A | Bos taurus |
| type II RNase A | isoform with higher molecular mass than commercial and type I RNase A | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
type I isoenzyme, Tm of 59°C. Type II isoenzyme, Tm of 53°C | Bos taurus |
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