Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.6.1.18 extracted from

  • Kadonosono, T.; Chatani, E.; Hayashi, R.; Moriyama, H.; Ueki, T.
    Minimization of cavity size ensures protein stability and folding: structures of Phe46-replaced bovine pancreatic RNase A (2003), Biochemistry, 42, 10651-10658.
    View publication on PubMed
Search for more literature for 4.6.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bos taurus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Bos taurus

Protein Variants

Protein Variants Comment Organism
F46A mutant with adversely affected conformational stability and folding speed Bos taurus
F46L mutant with adversely affected conformational stability and folding speed Bos taurus
F46V mutant with adversely affected conformational stability and folding speed Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.41
-
 cytidine-2',3'-cyclic monophosphate F46A mutant, pH 5.5, 25ºC Bos taurus
0.42
-
 cytidine-2',3'-cyclic monophosphate F46L mutant, pH 5.5, 25ºC Bos taurus
0.51
-
 cytidine-2',3'-cyclic monophosphate wild type enzyme, pH 5.5, 25ºC Bos taurus
0.64
-
 cytidine-2',3'-cyclic monophosphate F46V mutant, pH 5.5, 25ºC Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P61823
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytidine-2',3'-cyclic monophosphate + H2O hydrolysis reaction Bos taurus 3'-CMP
-
 ?