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Literature summary for 4.6.1.18 extracted from

  • Sorrentino, S.; Naddeo, M.; Russo, A.; D'Alessio, G.
    Degradation of double-stranded RNA by human pancreatic ribonuclease: Crucial Role of Noncatalytic Basic Amino Acid Residues (2003), Biochemistry, 42, 10182-10190.
    View publication on PubMed
Search for more literature for 4.6.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
G38D as active as wild type enzyme towards ssRNA, poly(C) and poly(U) single-stranded homopolymers. With 3fold reduction of the dsRNA degrading activity Homo sapiens
K102A as active as wild type enzyme towards ssRNA, poly(C) and poly(U) single-stranded homopolymers Homo sapiens
R4A as active as wild type enzyme towards ssRNA, poly(C) and poly(U) single-stranded homopolymers Homo sapiens
R4A/G38D/K102A ADA variant, triple mutant. This variant shows 62% and 83% activity on poly(C) and poly(U), respectively, with respect to wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 poly(A)poly(U) wild type enzyme Homo sapiens
0.209
-
 poly(A)poly(U) G38D mutant Homo sapiens
0.244
-
 poly(A)poly(U) ADA mutant Homo sapiens
0.314
-
 poly(A)poly(U) R4A mutant Homo sapiens
0.389
-
 poly(A)poly(U) K102A mutant Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P07998
-
-

Reaction

Reaction Comment Organism Reaction ID
an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA] specifically located basic residues, together with the lack of a negative charge and/or the presence of a glycine at position 38, may contribute to make the enzymatic degradation of the polyanionic double-helical substrate more efficient Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded RNA + H2O yeast dsRNA Homo sapiens 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
poly(A) + H2O poor substrate because of Thr45 in the substrate binding site of the enzyme that sterically excludes purine bases Homo sapiens 3'-AMP + 3'-oligonucleotides
-
 ?
poly(A)poly(U) + H2O
-
 Homo sapiens ?
-
 ?
poly(C) + H2O
-
 Homo sapiens 3'-CMP + 3'-phosphooligonucleotides
-
 ?
poly(U) + H2O
-
 Homo sapiens 3'-UMP + 3'-oligonucleotides
-
 ?
single-stranded RNA + H2O
-
 Homo sapiens ?
-
 ?