Literature summary for 4.6.1.18 extracted from

Pace, C.N.; Laurents, D.V.; Thomson, J.A.
pH Dependence of the urea and guanidine hydrochloride denaturation of ribonuclease A and ribonuclease T1 (1990), Biochemistry, 29, 2564-2572.

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Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
RNA + H2O	Bos taurus	endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA	3'-phosphomononucleotides + 3'-phosphooligonucleotides	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cyclic 2',3'-nucleoside monophosphate + H2O	second step of hydrolysis is irreversible	Bos taurus	3'-phosphomononucleotides	-	ir
RNA + H2O	endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA	Bos taurus	3'-phosphomononucleotides + 3'-phosphooligonucleotides	-	?
RNA + H2O	first step of hydrolysis is reversible	Bos taurus	cyclic 2',3'-nucleoside monophosphate	-	r

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
7	9	maximum conformational stability to urea and guanidine hydrochloride denaturation	Bos taurus

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Release 2023.1 (January 2023)