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Literature summary for 4.6.1.18 extracted from

  • Weickmann, J.L.; Elson, M.; Glitz, D.G.
    Purification and characterization of human pancreatic ribonuclease (1981), Biochemistry, 20, 1272-1278.
    View publication on PubMed
Search for more literature for 4.6.1.18

Activating Compound

Activating Compound Comment Organism Structure
NaCl
-
 Bos taurus
NaCl at 0.1-0.25 M Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
iodoacetate
-
 Homo sapiens
NaCl
-
 Bos taurus
NaCl above 0.25 M Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
 cyclic 2',3'-CMP
-
 Bos taurus
1
-
 cyclic 2',3'-CMP
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ NaCl: 0.1-0.25 M optimum concentration Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
-
 Bos taurus
15000
-
 pancreas, gel filtration, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA + H2O Homo sapiens endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
RNA + H2O Bos taurus endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Homo sapiens
-
pancreas
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1100
-
 brain, poly(C) Bos taurus
2400
-
 brain, RNA Bos taurus
10300
-
 RNA Homo sapiens
11300
-
 pancreas, RNA Bos taurus
22600
-
 pancreas, poly(C) Bos taurus
35000
-
 poly(C) Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclic 2',3'-nucleoside monophosphate + H2O second step of hydrolysis is irreversible Homo sapiens 3'-phosphomononucleotides
-
 ir
cyclic 2',3'-nucleoside monophosphate + H2O second step of hydrolysis is irreversible Bos taurus 3'-phosphomononucleotides
-
 ir
poly (C) + H2O
-
 Homo sapiens 3'-CMP + 3'-phosphooligonucleotides
-
 ?
poly (C) + H2O
-
 Bos taurus 3'-CMP + 3'-phosphooligonucleotides
-
 ?
RNA + H2O endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA Homo sapiens 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
RNA + H2O endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA Bos taurus 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
RNA + H2O first step of hydrolysis is reversible Homo sapiens cyclic 2',3'-nucleoside monophosphate
-
 r
RNA + H2O first step of hydrolysis is reversible Bos taurus cyclic 2',3'-nucleoside monophosphate
-
 r

Subunits

Subunits Comment Organism
monomer
-
 Bos taurus
monomer 1 * 15000, pancreas, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Homo sapiens