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Literature summary for 4.6.1.18 extracted from
- The ribonucleases: occurence, structure and properties (1961), The Enzymes, 2nd Ed. (Boyer, P. D. ; Lardy, H. ; Myrbäck, K. , eds. ), 5, 95-122.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| 2 M guanidine, activity remains | Bos taurus |
| 8 M urea, activity remains | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenite | - |
Bos taurus |  |
| chloride | - |
Bos taurus | |
| Selenite | - |
Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNA + H2O | Mus musculus | endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA | 3'-phosphomononucleotides + 3'-phosphooligonucleotides | - |
? | |
| RNA + H2O | Homo sapiens | endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA | 3'-phosphomononucleotides + 3'-phosphooligonucleotides | - |
? | |
| RNA + H2O | Rattus norvegicus | endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA | 3'-phosphomononucleotides + 3'-phosphooligonucleotides | - |
? | |
| RNA + H2O | Sus scrofa | endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA | 3'-phosphomononucleotides + 3'-phosphooligonucleotides | - |
? | |
| RNA + H2O | Bos taurus | endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA | 3'-phosphomononucleotides + 3'-phosphooligonucleotides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Mus musculus | - |
| pancreas | - |
Homo sapiens | - |
| pancreas | - |
Rattus norvegicus | - |
| pancreas | - |
Sus scrofa | - |
| pancreas | - |
Bos taurus | - |
| spleen | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyclic 2',3'-nucleoside monophosphate + H2O | second step of hydrolysis is irreversible | Mus musculus | 3'-phosphomononucleotides | - |
ir | |
| cyclic 2',3'-nucleoside monophosphate + H2O | second step of hydrolysis is irreversible | Homo sapiens | 3'-phosphomononucleotides | - |
ir | |
| cyclic 2',3'-nucleoside monophosphate + H2O | second step of hydrolysis is irreversible | Rattus norvegicus | 3'-phosphomononucleotides | - |
ir | |
| cyclic 2',3'-nucleoside monophosphate + H2O | second step of hydrolysis is irreversible | Sus scrofa | 3'-phosphomononucleotides | - |
ir | |
| cyclic 2',3'-nucleoside monophosphate + H2O | second step of hydrolysis is irreversible | Bos taurus | 3'-phosphomononucleotides | - |
ir | |
| RNA + H2O | endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA | Mus musculus | 3'-phosphomononucleotides + 3'-phosphooligonucleotides | - |
? | |
| RNA + H2O | endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA | Homo sapiens | 3'-phosphomononucleotides + 3'-phosphooligonucleotides | - |
? | |
| RNA + H2O | endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA | Rattus norvegicus | 3'-phosphomononucleotides + 3'-phosphooligonucleotides | - |
? | |
| RNA + H2O | endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA | Sus scrofa | 3'-phosphomononucleotides + 3'-phosphooligonucleotides | - |
? | |
| RNA + H2O | endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA | Bos taurus | 3'-phosphomononucleotides + 3'-phosphooligonucleotides | - |
? | |
| RNA + H2O | first step of hydrolysis is reversible | Mus musculus | cyclic 2',3'-nucleoside monophosphate | - |
r | |
| RNA + H2O | first step of hydrolysis is reversible | Homo sapiens | cyclic 2',3'-nucleoside monophosphate | - |
r | |
| RNA + H2O | first step of hydrolysis is reversible | Rattus norvegicus | cyclic 2',3'-nucleoside monophosphate | - |
r | |
| RNA + H2O | first step of hydrolysis is reversible | Sus scrofa | cyclic 2',3'-nucleoside monophosphate | - |
r | |
| RNA + H2O | first step of hydrolysis is reversible | Bos taurus | cyclic 2',3'-nucleoside monophosphate | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Rattus norvegicus |
| 7 | 7.5 | - |
Bos taurus |
| 7 | - |
- |
Mus musculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.8 | - |
Homo sapiens |
| 7 | 7.8 | - |
Rattus norvegicus |
| 7 | 7.8 | - |
Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
activity remains below pH 2 | Bos taurus |
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