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Literature summary for 4.6.1.18 extracted from

  • Richards, F.M.; Wyckoff, H.W.
    Bovine pancreatc ribonuclease (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 647-806.
No PubMed abstract available
Search for more literature for 4.6.1.18

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Bos taurus

General Stability

General Stability Organism
-20°C, phosphate buffer, no salts stabilize Bos taurus
Cu2+, binding of Cu2+ lowers stability to thermal and urea denaturation Bos taurus
urea, denaturation, urea interferes with interhydrophobic interactions by affecting the water molecules Bos taurus
Zn2+, binding of Zn2+ lowers stability to thermal and urea denaturation Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
2'(3')Nucleotides
-
 Bos taurus
2'-Deoxynucleotides
-
 Bos taurus
5-aminoethyluracil
-
 Bos taurus
5-Nitrouracil
-
 Bos taurus
adenosine 5'-phosphate
-
 Bos taurus
BeCl2
-
 Bos taurus
Copolymer of glutamic acid and tyrosine
-
 Bos taurus
cytidine
-
 Bos taurus
Hydrobenzoinphosphate
-
 Bos taurus
Mercury hematoporphyrin
-
 Bos taurus
oligonucleotides e.g. ApUp Bos taurus
penicillin
-
 Bos taurus
Phenylphosphate
-
 Bos taurus
PO43-
-
 Bos taurus
Polyanions natural and synthetic, free poly(A), poly(U) Bos taurus
putrescine
-
 Bos taurus
vitamin B12
-
 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ enhance interaction with substrate but lower stability Bos taurus
Ni2+
-
 Bos taurus
Zn2+ enhance interaction with substrate but lower stability Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13680
-
 pancreas, amino acid composition Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA + H2O Bos taurus endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information thermal transition temperature in various solvents Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Bos taurus

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CpA + H2O
-
 Bos taurus adenosine + 3'-CMP
-
 ?
CpG + H2O
-
 Bos taurus guanosine + 3'-CMP
-
 ?
cyclic 2',3'-cytidine monophosphate + H2O
-
 Bos taurus 3'-CMP
-
 ir
cyclic 2',3'-nucleoside monophosphate + H2O second step of hydrolysis is irreversible Bos taurus 3'-phosphomononucleotides
-
 ir
poly (C) + H2O
-
 Bos taurus 3'-CMP + 3'-phosphooligonucleotides
-
 ?
RNA + H2O endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA Bos taurus 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
RNA + H2O first step of hydrolysis is reversible Bos taurus cyclic 2',3'-nucleoside monophosphate
-
 r
UpA + H2O
-
 Bos taurus adenosine + 3'-UMP
-
 ?
UpG + H2O
-
 Bos taurus guanosine + 3'-UMP
-
 ?