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Literature summary for 4.6.1.16 extracted from

  • Fujishima, K.; Sugahara, J.; Miller, C.S.; Baker, B.J.; Di Giulio, M.; Takesue, K.; Sato, A.; Tomita, M.; Banfield, J.F.; Kanai, A.
    A novel three-unit tRNA splicing endonuclease found in ultrasmall Archaea possesses broad substrate specificity (2011), Nucleic Acids Res., 39, 9695-9704.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.16

Cloned(Commentary)

Cloned (Comment) Organism
epsilon2 endonuclease, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of C-terminally His6-tagged wild-type and mutant enzyme Candidatus Micrarchaeum acidiphilum

Protein Variants

Protein Variants Comment Organism
D357A site-directed mutagenesis Candidatus Micrarchaeum acidiphilum
K224E site-directed mutagenesis Candidatus Micrarchaeum acidiphilum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Candidatus Micrarchaeum acidiphilum

Organism

Organism UniProt Comment Textmining
Candidatus Micrarchaeum acidiphilum C7DIA5 ARMAN-2
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information epsilon2 endonuclease cleaves both canonical and noncanonical bulge-helix-bulge motifs, BHB motif, both strict and relaxed Candidatus Micrarchaeum acidiphilum ?
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 ?

Subunits

Subunits Comment Organism
homodimer the enzyme consists of two duplicated catalytic units and one structural unit encoded on a single gene, representing a three-unit architecture, the conserved L10-pocket interaction between catalytic and structural unit is necessary for the assembly, protein domain structure, overview Candidatus Micrarchaeum acidiphilum

Synonyms

Synonyms Comment Organism
epsilon2 endonuclease
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 Candidatus Micrarchaeum acidiphilum
More fourth type of archaeal tRNA splicing endonuclease Candidatus Micrarchaeum acidiphilum
three-unit tRNA splicing endonuclease
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 Candidatus Micrarchaeum acidiphilum
tRNA splicing endonuclease
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 Candidatus Micrarchaeum acidiphilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
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 assay at Candidatus Micrarchaeum acidiphilum

General Information

General Information Comment Organism
evolution in Archaea, three types of splicing endonuclease are produced, homotetrameric: alpha4, homodimeric: alpha2, and heterotetrameric: (alphabeta)2. epsilon2 endonuclease represents a fourth type: epsilon2. The presence of an epsilon2 endonuclease in an archaeon deeply branched within Euryarchaeota represents an example of the coevolution of tRNA and their processing enzymes. The ancestral alpha4 endonuclease is assumed to have undergone two independent gene duplication events giving rise to alpha2 and (alphabeta)2 architecture via subfunctionalization, while most of the (alphabeta)2 endonucleases and korarchaeal a2 endonuclease have acquired extra insertion sequences that interact with BHB motif to broaden their substrate specificity. Transition of splicing endonuclease from alpha4 to epsilon2 type, overview Candidatus Micrarchaeum acidiphilum
physiological function tRNA splicing endonucleases are involved in the processing of pre-tRNA molecules Candidatus Micrarchaeum acidiphilum