Cloned (Comment) | Organism |
---|---|
genes endo1 and endo2, expression as His-tagged subunits forming the heterocomplex | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
catalytic subunit, X-ray diffraction structure determination and analysis at 3.1 A resolution | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
H136A | site-directed mutagenesis of the catalytically essential residue results in an inactive mutant | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharolobus solfataricus | the enzyme is responsible for recognition and excision of nuclear tRNA and all archeal introns | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
genes endo1 and endo2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged subunits by nickel affinity chromatography | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is responsible for recognition and excision of nuclear tRNA and all archeal introns | Saccharolobus solfataricus | ? | - |
? | |
additional information | the enzyme of Sulfolobus solfataricus shows a broader substrate specificity | Saccharolobus solfataricus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | heterodimeric enzyme with subunit encoded by genes endo1 and endo2, analysis of the quarternary structure, structure of catalytic subunit, overview | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
RNA splicing endonuclease | - |
Saccharolobus solfataricus |