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Literature summary for 4.6.1.14 extracted from
- Roles of Gln81 and Cys80 in catalysis by glycosylphosphatidylinositol-phospholipase C from Trypanosoma brucei (1999), Eur. J. Biochem., 264, 914-920.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C184A | activity is not effected | Trypanosoma brucei |
| C269-270-273S | 33% activity of wild-type enzyme | Trypanosoma brucei |
| C80A | 33% activity of wild-type enzyme, resistant to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
| C80F | enzyme is inactive | Trypanosoma brucei |
| C80T | 33% activity of wild-type enzyme, resistant to p-chloromercuriphenylsulfonic acid | Trypanosoma brucei |
| Q81A | enzyme is inactive | Trypanosoma brucei |
| Q81E | enzyme is inactive | Trypanosoma brucei |
| Q81G | enzyme is inactive | Trypanosoma brucei |
| Q81K | enzyme is inactive | Trypanosoma brucei |
| Q81L | enzyme is inactive | Trypanosoma brucei |
| Q81N | specific activity is 500fold decreased | Trypanosoma brucei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0003 | - |
myristate-labeled variant-surface-glycoprotein | 37°C, pH 8, 50 mM sodium phosphate, Q81N | Trypanosoma brucei | |
| 0.0005 | - |
myristate-labeled variant-surface-glycoprotein | 37°C, pH 8, 50 mM sodium phosphate, wild-type | Trypanosoma brucei | |
| 0.0011 | - |
myristate-labeled variant-surface-glycoprotein | 37°C, pH 8, wild-type | Trypanosoma brucei | |
| 0.0018 | - |
myristate-labeled variant-surface-glycoprotein | 37°C, pH 8, C80A | Trypanosoma brucei | |
| 0.0021 | - |
myristate-labeled variant-surface-glycoprotein | 37°C, pH 8, C80T | Trypanosoma brucei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2000 | - |
Q81E | Trypanosoma brucei |
| 9000 | - |
Q81N | Trypanosoma brucei |
| 280000 | - |
C80A | Trypanosoma brucei |
| 1100000 | - |
wild-type | Trypanosoma brucei |
| 3500000 | - |
C80T | Trypanosoma brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myristate-labeled variant-surface-glycoprotein | - |
Trypanosoma brucei | ? | - |
? |  |
| variant-surface-glycoprotein | - |
Trypanosoma brucei | 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein | - |
? | |
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