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Literature summary for 4.6.1.14 extracted from

  • Fox, J.A.; Duszenko, M.; Ferguson, M.A.J.; Low, M.G.; Cross, G.A.M.
    Purification and characterization of a novel glycan-phosphatidylinositol-specific phospholipase C from Trypanosoma brucei (1986), J. Biol. Chem., 261, 15767-15771.
    View publication on PubMed
Search for more literature for 4.6.1.14

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol enzyme activity shows marked stimulation in the presence of the thiol-reducing agent, DTT Trypanosoma brucei
EGTA
-
 Trypanosoma brucei

General Stability

General Stability Organism
GPI-PLC is very stable, maintaining full activity at 4°C for 1-2 months Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ activity of GPI-PLC exhibited small but significant inhibition in the presence of calcium ions, 70% activity left Trypanosoma brucei
Hg2+ 10 microM 100% inhibition, 1 microM 67% activity left Trypanosoma brucei
Zn2+ 100 microM 100% inhibition, 10 microM 59% activity left Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane GPI-PLC is an integral membrane protein Trypanosoma brucei 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Hg2+ 10 microM 100% inhibition, 1 microM 67% activity left Trypanosoma brucei
additional information calcium-independent Trypanosoma brucei
Zn2+ 100 microM 100% inhibition, 10 microM 59% activity left Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39800
-
 SDS-PAGE, visualized either by Coomassie Blue or by silver staining Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trypanosoma brucei enzyme exhibits substantial specificity for molecules containing glycosylated phosphatidylinositol ?
-
 ?
additional information Trypanosoma brucei 427 enzyme exhibits substantial specificity for molecules containing glycosylated phosphatidylinositol ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-
Trypanosoma brucei 427
-
-
-

Purification (Commentary)

Purification (Comment) Organism
anion exchange chromatography on DEAE-cellulose followed by chromatography on phosphatidylinositol-Sepharose Trypanosoma brucei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00245
-
 last purification step, PI-Sepharose Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimyristyl-P + H2O
-
 Trypanosoma brucei ?
-
 ?
glycosylated phosphatidylinositol + H2O
-
 Trypanosoma brucei ?
-
 ?
membrane form variant surface glycoprotein + H2O mfVSG, enzyme catalyzes the conversion of membrane form variant surface glycoproteins to soluble variant surface glycoproteins, with the release of sn-1,2-dimyristylglycerol Trypanosoma brucei soluble variant surface glycoprotein + sn-1,2-dimyristoylglycerol
-
 ?
additional information enzyme exhibits substantial specificity for molecules containing glycosylated phosphatidylinositol Trypanosoma brucei ?
-
 ?
additional information [32P]phosphatidylinositol-4-phosphate, [32P]phosphatidylinositol 4,5-bisphosphate or [3H]phosphatidylcholine are not significantly hydrolyzed by the GPI-PLC Trypanosoma brucei ?
-
 ?
additional information enzyme exhibits substantial specificity for molecules containing glycosylated phosphatidylinositol Trypanosoma brucei 427 ?
-
 ?
additional information [32P]phosphatidylinositol-4-phosphate, [32P]phosphatidylinositol 4,5-bisphosphate or [3H]phosphatidylcholine are not significantly hydrolyzed by the GPI-PLC Trypanosoma brucei 427 ?
-
 ?
pronase fragment + H2O aspartylethanolamine-glycolipid moiety of mfVSG Trypanosoma brucei ?
-
 ?
pronase fragment of mfVSG + H2O contains only the terminal amino acid and the glycolipid anchor Trypanosoma brucei ?
-
 ?

Subunits

Subunits Comment Organism
? x * 39800, SDS-PAGE Trypanosoma brucei

Synonyms

Synonyms Comment Organism
glycan-phosphatidylinositol-specific phospholipase C
-
 Trypanosoma brucei
GPI-PC
-
 Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
 two maxima, one at pH 7.0 and the other at pH 10.0, that shows the highest enzyme activity Trypanosoma brucei

pH Range

pH Minimum pH Maximum Comment Organism
7 10 pH dependence of GPI-PLC activity. Two maxima, one at pH 7.0 and the other at pH 10.0, that shows the highest enzyme activity Trypanosoma brucei