Literature summary for 4.6.1.12 extracted from

Rohdich, F.; Eisenreich, W.; Wungsintaweekul, J.; Hecht, S.; Schuhr, C.A.; Bacher, A.
Biosynthesis of terpenoids. 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) from Plasmodium falciparum (2001), Eur. J. Biochem., 268, 3190-3197.

Search for more literature for 4.6.1.12

Application

Application	Comment	Organism
medicine	the enzyme is a potential target for antimalarial drugs directed at the nonmevalonate pathway of isoprenoid biosynthesis	Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Plasmodium falciparum

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	5 mM. Activity can be restored by the addition of Mg2+ to a concentration of 10 mM	Plasmodium falciparum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.252	-	4-diphosphocytidyl-2C-methyl-D-erythritol	pH 7.0, 27°C	Plasmodium falciparum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	Mg2+ or Mn2+ are required	Plasmodium falciparum
Mn2+	Mg2+ or Mn2+ are required	Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
21348	-	x * 21348, electrospray mass spectrometry	Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-phospho-4-(cytidine 5'-diphospho)-2C-methyl-D-erythritol	Plasmodium falciparum	nonmevalonate pathway of isoprenoid biosynthesis	2C-methyl-D-erythritol-2,4-cyclodiphosphate + CMP	-	?

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	P62368	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.75	-	-	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-4-(cytidine 5'-diphospho)-2C-methyl-D-erythritol	-	Plasmodium falciparum	2C-methyl-D-erythritol-2,4-cyclodiphosphate + CMP	-	?
2-phospho-4-(cytidine 5'-diphospho)-2C-methyl-D-erythritol	at low rate	Plasmodium falciparum	2C-methyl-D-erythritol-2,4-cyclodiphosphate + CMP	-	?
2-phospho-4-(cytidine 5'-diphospho)-2C-methyl-D-erythritol	nonmevalonate pathway of isoprenoid biosynthesis	Plasmodium falciparum	2C-methyl-D-erythritol-2,4-cyclodiphosphate + CMP	-	?
4-diphosphocytidyl-2C-methyl-D-erythritol	at low rate	Plasmodium falciparum	2C-methyl-D-erythritol 3,4-cyclophosphate + CMP	-	?

Subunits

Subunits	Comment	Organism
?	x * 21348, electrospray mass spectrometry	Plasmodium falciparum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Plasmodium falciparum

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Release 2023.1 (January 2023)