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Literature summary for 4.6.1.1 extracted from

  • Penzkofer, A.; Tanwar, M.; Veetil, S.; Kateriya, S.
    Photo-dynamics of photoactivated adenylyl cyclase TpPAC from the spirochete bacterium Turneriella parva strain HT (2015), J. Photochem. Photobiol. B, 153, 90-102 .
    View publication on PubMed
Search for more literature for 4.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Turneriella parva

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Turneriella parva
-
-

Organism

Organism UniProt Comment Textmining
Turneriella parva
-
-
-
Turneriella parva DSM 21527
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Turneriella parva

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
53.3
-
 irradiation with blue light, peak wavelength 460 nm, pH 7.5, 23°C Turneriella parva

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP
-
 Turneriella parva 3',5'-cAMP + diphosphate
-
 ?
ATP
-
 Turneriella parva DSM 21527 3',5'-cAMP + diphosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
46
-
 melting temperature Turneriella parva

General Information

General Information Comment Organism
physiological function PAC shows adenylyl cyclase activity of ATP to cAMP conversion highly stimulated by blue-light exposure and low adenylyl cyclase activity in the dark. Photoexcitation of PAC in its light-adapted signaling state (BLUFs, secondary BLUF domain photocycling) causes high-efficient fast photo-induced Tyr to flavin electron transfer and charge recombination with weak permanent flavin cofactor degradation likely to covalent binding of reduced flavin to protein Turneriella parva