Literature summary for 4.6.1.1 extracted from

Fulcher, N.B.; Holliday, P.M.; Klem, E.; Cann, M.J.; Wolfgang, M.C.
The Pseudomonas aeruginosa Chp chemosensory system regulates intracellular cAMP levels by modulating adenylate cyclase activity (2010), Mol. Microbiol., 76, 889-904.

Search for more literature for 4.6.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
the putative catalytic domain (amino acids 217-463) as an N-terminal hexahistidine fusion is expressed in Escherichia coli M15[pREP4] cells	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
K274A	the mutation significantly reduces adenylate cyclase activity	Pseudomonas aeruginosa
T351A	the mutation significantly reduces adenylate cyclase activity	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0667	-	ATP	pH 8.5, 40°C	Pseudomonas aeruginosa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Pseudomonas aeruginosa
Mn2+	required, the enzyme has higher specific activity for Mn2+-ATP versus Mg2+-ATP	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-
Pseudomonas aeruginosa PAK	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA affinity resin column chromatography	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP	-	Pseudomonas aeruginosa	3',5'-cyclic-AMP + diphosphate	-	?
ATP	-	Pseudomonas aeruginosa PAK	3',5'-cyclic-AMP + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
CyaB	-	Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Pseudomonas aeruginosa

Cofactor

Cofactor	Comment	Organism	Structure
Calmodulin	-	Pseudomonas aeruginosa

General Information

General Information	Comment	Organism
physiological function	the Chp chemotaxis-like system regulates intracellular cAMP levels by modulating CyaB activity	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)