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Literature summary for 4.6.1.1 extracted from

  • Guo, Y.L.; Mayer, H.; Vollmer, W.; Dittrich, D.; Sander, P.; Schultz, A.; Schultz, J.E.
    Polyphosphates from Mycobacterium bovis--potent inhibitors of class III adenylate cyclases (2009), FEBS J., 276, 1094-1103.
    View publication on PubMed
Search for more literature for 4.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)(pRep4) cells Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
cyclic poly-phosphate 17
-
 Mycobacterium tuberculosis
cyclic triphosphate
-
 Mycobacterium tuberculosis
diphosphate
-
 Mycobacterium tuberculosis
additional information class I AC is unaffected by up to 0.03 mM poly-phosphate 75 Mycobacterium tuberculosis
phosphate
-
 Mycobacterium tuberculosis
poly-phosphate 16
-
 Mycobacterium tuberculosis
poly-phosphate 25
-
 Mycobacterium tuberculosis
poly-phosphate 30
-
 Mycobacterium tuberculosis
poly-phosphate 45
-
 Mycobacterium tuberculosis
poly-phosphate 68
-
 Mycobacterium tuberculosis
poly-phosphate 75
-
 Mycobacterium tuberculosis
Triphosphate
-
 Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mycobacterium tuberculosis 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP Mycobacterium tuberculosis
-
 3',5'-cyclic-AMP + diphosphate
-
 ?
ATP Mycobacterium tuberculosis BCG 1721
-
 3',5'-cyclic-AMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis BCG 1721
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP
-
 Mycobacterium tuberculosis 3',5'-cyclic-AMP + diphosphate
-
 ?
ATP
-
 Mycobacterium tuberculosis BCG 1721 3',5'-cyclic-AMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
AC Rv1625c
-
 Mycobacterium tuberculosis
class III adenylate cyclase
-
 Mycobacterium tuberculosis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000011
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis poly-phosphate 75
0.000012
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis poly-phosphate 45
0.000012
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis poly-phosphate 68
0.000018
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis poly-phosphate 30
0.00002
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis Triphosphate
0.000027
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis poly-phosphate 25
0.000033
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis cyclic poly-phosphate 17
0.0001
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis poly-phosphate 16
0.00021
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis diphosphate
0.002
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis cyclic triphosphate
11
-
 recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), at 30°C Mycobacterium tuberculosis phosphate