Literature summary for 4.6.1.1 extracted from

Sakihama, T.; Masuda, K.; Sato, T.; Doi, T.; Kodama, T.; Hamakubo, T.
Functional reconstitution of G-protein-coupled receptor-mediated adenylyl cyclase activation by a baculoviral co-display system (2008), J. Biotechnol., 135, 28-33.

Activating Compound

Activating Compound	Comment	Organism	Structure
stimulating G protein	GalphaS, and Gbeta1 and Ggamma2 subunits, G-protein-coupled receptors couple Galphas and activate isozyme ACVI	Homo sapiens

Application

Application	Comment	Organism
analysis	the enzyme activity is useful as a marker for determination of G-protein-coupled receptor function mediating adenylyl cylcase activation by extracellular baculovirus particles, usage of this budded virus display system to detect G-protein-coupled receptor signaling, method development, overview	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression of isozyme ACVI in Spodoptera frugiperda Sf9 cell membranes, with a combination of four recombinant baculoviruses individually encoding the dopamine D1 receptor, G-protein alphaS-subunit, G-protein beta1gamma2 subunit dimer, and adenylyl cyclase type VI, ACVI. The recovered budded virus fraction produces cAMP in response to the stimulation with dopamine. Co-expression of all three G-protein subunits inaddition to receptor and ACVI led to amaximal response. Budded virusses co-expressingthel proteins also respond to dopamine agonists fenoldopam and SKF38393 and the antagonist flupenthixol, overview	Homo sapiens

Cloned (Comment)

Organism

expression of isozyme ACVI in Spodoptera frugiperda Sf9 cell membranes, with a combination of four recombinant baculoviruses individually encoding the dopamine D1 receptor, G-protein alphaS-subunit, G-protein beta1gamma2 subunit dimer, and adenylyl cyclase type VI, ACVI. The recovered budded virus fraction produces cAMP in response to the stimulation with dopamine. Co-expression of all three G-protein subunits inaddition to receptor and ACVI led to amaximal response. Budded virusses co-expressingthel proteins also respond to dopamine agonists fenoldopam and SKF38393 and the antagonist flupenthixol, overview

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
inhibitory G protein	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Homo sapiens	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP	Homo sapiens	-	3',5'-cyclic-AMP + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O43306	isozyme ACVI	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP	-	Homo sapiens	3',5'-cyclic-AMP + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
ACVI	-	Homo sapiens
adenylyl cyclase type VI	-	Homo sapiens